Kornfehl J, Neuchrist C, Grasl M C, Piza H, Roka R, Ehrenberger K, Sorg C, Kraft D, Scheiner O
Institute of General and Experimental Pathology, University of Vienna, Austria.
Immunobiology. 1992 Apr;184(4-5):321-35. doi: 10.1016/S0171-2985(11)80590-5.
Autologous jejunum, transplanted as a functional replacement immediately after radical dissection of advanced stages of squamous cell carcinomas of the head and neck and subsequently irradiated, was examined by immunohistochemistry (APAAP/PAP-technique). Biopsies from 9 patients were taken at the time of transplantation and up to 24 months thereafter (group 1) and from 5 patients only once after transplantation (group 2). Twenty-six monoclonal antibodies (mAbs) were used as surface markers to give an overview about phenotypical changes with respect to T-, B- and M phi-antigens. 1) B cells: a general increase of CR2+ (CD21, p less than 0.01) could be noticed after transplantation, immunoglobulin positive cells remained unchanged expect for a significant decrease of IgM+ (p less than 0.01) and IgA1+ (p less than 0.01) cells. 2) The number of T cells (CD3+) showed no significant differences although TcR gamma/delta+ cells decreased (p less than 0.01) in the autotransplant. ICAM-1 (CD54) and IL-2R (CD25) were found on a significant (p less than 0.01) higher number of cells after transplantation. 3) Cells with M/M phi morphology showed increased expression of the Fc gamma receptors (CD64, p less than 0.001; CD32, n.s.; CD16, p less than 0.001), of the complement receptors CR1 (CD35, (p less than 0.001) and CR3 (CD11b, p less than 0.02), of HLA-DQ (p less than 0.01), and of the antigens 25F9 (mature M phi; p less than 0.01) and CD4 (p less than 0.02). Correlation analyses of data obtained from the biopsies of the 14 autotransplanted jejunum cases revealed a CD35+ and a 25F9+ subpopulation of M/M phi. Our findings indicate that despite irradiation autotransplanted jejunum contained cells with immunological capacities. Therefore, the replacement of larynx by autologous jejunum may facilitate not only mechanical but also immunological functions.
对头颈部鳞状细胞癌晚期进行根治性切除后，立即将自体空肠作为功能性替代物进行移植，并随后进行放疗，采用免疫组织化学方法（APAAP/PAP技术）对其进行检查。在移植时及此后长达24个月的时间里，从9例患者身上获取活检样本（第1组），从5例患者身上仅在移植后获取一次活检样本（第2组）。使用26种单克隆抗体（mAb）作为表面标志物，以全面了解T、B和Mφ抗原方面的表型变化。1）B细胞：移植后可观察到CR2+（CD21，p<0.01）普遍增加，免疫球蛋白阳性细胞除IgM+（p<0.01）和IgA1+（p<0.01）细胞显著减少外保持不变。2）T细胞（CD3+）数量无显著差异，尽管自体移植中空肠中TcRγ/δ+细胞减少（p<0.01）。移植后发现ICAM-1（CD54）和IL-2R（CD25）在显著更多的细胞上表达（p<0.01）。3）具有M/Mφ形态的细胞显示Fcγ受体（CD64，p<0.001；CD32，无显著差异；CD16，p<0.001）、补体受体CR1（CD35，（p<0.001）和CR3（CD11b，p<0.02）、HLA-DQ（p<0.01）以及抗原25F9（成熟Mφ；p<0.01）和CD4（p<0.02）的表达增加。对14例自体移植空肠病例活检数据的相关分析揭示了M/Mφ的CD35+和25F9+亚群。我们的研究结果表明，尽管经过放疗，自体移植空肠仍含有具有免疫能力的细胞。因此，用自体空肠替代喉不仅可能促进机械功能，还可能促进免疫功能。
