Kornfehl J, Neuchrist C, Grasl M C, Ehrenberger K, Kraft D, Scheiner O
1st ENT Department, University of Vienna, Austria.
Eur Arch Otorhinolaryngol. 1993;250(3):168-72. doi: 10.1007/BF00171705.
The expressions and cellular distributions of two pairs of adhesion molecules CD2/LFA-3 (leukocyte function-associated antigen-3) and LFA-1/ICAM-1 (intercellular adhesion molecule-1) were examined in inflammatory cellular infiltrates of advanced squamous cell carcinomas of the head and neck by immunohistochemical techniques including double-staining methods. Thirteen patients were investigated using the following monoclonal antibodies (mAbs): CD2, LFA-3 (CD58), ICAM-1 (CD54), LFA-1 (CD11a), the alpha/beta and gamma/delta T-cell receptor, pan T cells and broadly distributed monocyte/macrophage (m/m phi) [Fc gamma RII (CD32), 25F9, RM3/1]. LFA-3 staining was observed on a high number of cells (968 +/- 112 cells/mm2), correlating to the number of Fc gamma RII (CD32; P < 0.01), 25F9 (P < 0.05) and RM3/1 (P < 0.05) positive m/m phi. Its ligand CD2 was found on 365 +/- 126 cells/mm2, representing about 50% of CD3+ cells (730 +/- 286 cells/mm2). CD2 positivity correlated to CD3 and CD8 (P < 0.01) but not to CD4+ T cells. LFA-1 and ICAM-1 were expressed on lymphocytes as well as on m/m phi. ICAM-1+ cells (902 +/- 205 cells/mm2) correlated to CD3+, CD8+ and RM3/1+ cells (P < 0.01). LFA-1 positivity (803 +/- 255 cells/mm2) showed correlations to nearly all investigated antigens, as well as to CD4+ T cells (P < 0.05). These results show that different m/m phi subsets display distinct patterns of adhesion molecule expressions suggesting different pathways of regulation. The CD3+ lymphocyte population revealed a lack of CD2 expression that was more pronounced in the CD4+ subset and indicated impaired lymphocyte function.
采用包括双重染色法在内的免疫组织化学技术，对头颈部晚期鳞状细胞癌炎症细胞浸润中两对黏附分子CD2/LFA - 3（白细胞功能相关抗原 - 3）和LFA - 1/ICAM - 1（细胞间黏附分子 - 1）的表达及细胞分布进行了检测。使用以下单克隆抗体（mAb）对13例患者进行了研究：CD2、LFA - 3（CD58）、ICAM - 1（CD54）、LFA - 1（CD11a）、α/β和γ/δ T细胞受体、全T细胞以及广泛分布的单核细胞/巨噬细胞（m/m phi）[FcγRII（CD32）、25F9、RM3/1]。在大量细胞（968±112个细胞/mm²）上观察到LFA - 3染色，其与FcγRII（CD32；P < 0.01）、25F9（P < 0.05）和RM3/1（P < 0.05）阳性的m/m phi数量相关。在365±126个细胞/mm²上发现其配体CD2，约占CD3⁺细胞（730±286个细胞/mm²）的50%。CD2阳性与CD3和CD8相关（P < 0.01），但与CD4⁺ T细胞无关。LFA - 1和ICAM - 1在淋巴细胞以及m/m phi上均有表达。ICAM - 1⁺细胞（902±205个细胞/mm²）与CD3⁺、CD8⁺和RM3/1⁺细胞相关（P < 0.01）。LFA - 1阳性（803±255个细胞/mm²）与几乎所有研究的抗原以及CD4⁺ T细胞均相关（P < 0.05）。这些结果表明，不同的m/m phi亚群表现出不同的黏附分子表达模式，提示存在不同的调节途径。CD3⁺淋巴细胞群体显示出CD2表达缺失，在CD4⁺亚群中更为明显，表明淋巴细胞功能受损。
