在美国，通过使用全细胞和重组抗原的酶联免疫吸附测定法检测自然感染伯氏疏螺旋体的马匹中的抗体。
Detection of antibodies to Borrelia burgdorferi in naturally infected horses in the USA by enzyme-linked immunosorbent assay using whole-cell and recombinant antigens.
作者信息
Magnarelli L, Fikrig E
机构信息
The Connecticut Agricultural Experiment Station, P.O. Box 1106, New Haven, CT 06504, USA.
出版信息
Res Vet Sci. 2005 Oct;79(2):99-103. doi: 10.1016/j.rvsc.2004.11.009. Epub 2005 Feb 9.
Blood samples were collected from 98 horses suspected of having borreliosis or granulocytic ehrlichiosis in Connecticut and New York State, USA during 1985, 1995, and 1996. Serum antibodies to Borrelia burgdorferi were detected by an enzyme-linked immunosorbent assay (ELISA), based on whole-cell and recombinant antigens, in 82 (84%) horses. Of the 181 sera tested, 59% were positive, using whole-cell antigens, compared to 48% with protein (p)37 and 35% with VlsE antigens. An ELISA containing either of these fusion proteins can be used as an adjunct to general screening by an ELISA or immunoblotting in animals not vaccinated for this disease.
1985年、1995年和1996年期间，从美国康涅狄格州和纽约州98匹疑似感染莱姆病或粒细胞埃立克体病的马身上采集血样。基于全细胞和重组抗原，采用酶联免疫吸附测定（ELISA）法检测了82匹（84%）马血清中抗伯氏疏螺旋体的抗体。在检测的181份血清中，使用全细胞抗原时59%呈阳性，相比之下，使用p37蛋白时为48%，使用VlsE抗原时为35%。含有这些融合蛋白之一的ELISA可作为未接种该疾病疫苗动物进行ELISA或免疫印迹一般筛查的辅助手段。
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