用于检测马属动物中抗伯氏疏螺旋体抗体的类特异性和多价酶联免疫吸附测定法。
Class-specific and polyvalent enzyme-linked immunosorbent assays for detection of antibodies to Borrelia burgdorferi in equids.
作者信息
Magnarelli L A, Anderson J F
机构信息
Department of Entomology, Connecticut Agricultural Experiment Station, New Haven 06504.
出版信息
J Am Vet Med Assoc. 1989 Nov 15;195(10):1365-8.
Class-specific and polyvalent ELISA were developed to detect IgM antibody or total immunoglobulins to Borrelia burgdorferi in equine sera. Analyses of 122 serum specimens, collected during 1985 from horses and ponies in tick-infested areas of Connecticut, revealed IgM antibody in 41 (34%) samples; titration end points ranged from 1:160 to 1:2,560. In polyvalent ELISA, 73 (16%) of 454 serum specimens contained IgM and/or IgG antibody. Seropositivity was highest (32%) for blood samples collected during May. Both ELISA procedures had comparable sensitivities.
开发了针对特定类别和多价的酶联免疫吸附测定（ELISA），以检测马血清中针对伯氏疏螺旋体的IgM抗体或总免疫球蛋白。对1985年从康涅狄格州蜱虫滋生地区的马和小马采集的122份血清样本进行分析，发现41份（34%）样本中有IgM抗体；滴定终点范围为1:160至1:2560。在多价ELISA中，454份血清样本中有73份（16%）含有IgM和/或IgG抗体。5月份采集的血液样本血清阳性率最高（32%）。两种ELISA方法具有相当的灵敏度。
Zotero 插件