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采用固定化脂质体色谱法分离中药复方中与脂质体膜相互作用的化合物。

Separation of compounds interacting with liposome membrane in combined prescription of traditional Chinese medicines with immobilized liposome chromatography.

作者信息

Sheng Liang-Hong, Li Song-Lin, Kong Liang, Chen Xue-Guo, Mao Xi-Qin, Su Xing-Ye, Zou Han-Fa, Li Ping

机构信息

National Chromatographic R&A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 161 Zhongshan Road, Dalian 116011, China.

出版信息

J Pharm Biomed Anal. 2005 Jun 15;38(2):216-24. doi: 10.1016/j.jpba.2005.01.008.

DOI:10.1016/j.jpba.2005.01.008
PMID:15925211
Abstract

Immobilized liposome chromatography (ILC), the stationary phase of which has been regarded as a mimic biomembranes system was used to separate and analyze compounds interacting with liposome membrane in Danggui Buxue decoction, a combined prescription of traditional Chinese medicines (CPTCMs), and its compositions Radix Astragli and Radix Angelica Sinensis. More than 10 main peaks in the extract of Danggui Buxue decoction were resolved on the ILC column, suggesting that more than 10 components in the prescription have significant retention on ILC column. Ligustilide, astragaloside IV and formononetin, three main bioactive ingredients in Danggui Buxue decoction, were found to have relatively significant, while ferulic acid, another bioactive ingredient in the prescription, relatively weak retention on ILC column. Effects of the eluent pH and amount of immobilized phosphatidylcholine (PC) on separation of interactional compounds in the extract of Danggui Buxue decoction were also investigated. It was found that these two factors strongly affected the retention of some interactional compounds. In addition, the fractions partitioned with different solvents from water extract of this combined prescription were evaluated with this ILC column system.

摘要

固定化脂质体色谱法(ILC),其固定相被视为一种模拟生物膜系统,用于分离和分析与当归补血汤(一种中药复方)及其组成成分黄芪和当归中与脂质体膜相互作用的化合物。当归补血汤提取物在ILC柱上分离出10多个主峰,表明该方剂中10多种成分在ILC柱上有显著保留。当归补血汤中的三种主要生物活性成分藁本内酯、黄芪甲苷和芒柄花素在ILC柱上有相对显著的保留,而该方剂中的另一种生物活性成分阿魏酸在ILC柱上的保留相对较弱。还研究了洗脱液pH值和固定化磷脂酰胆碱(PC)用量对当归补血汤提取物中相互作用化合物分离的影响。发现这两个因素强烈影响一些相互作用化合物的保留。此外,用该ILC柱系统对该复方水提取物用不同溶剂分配得到的馏分进行了评价。

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