Kim Yong-Mu, Jeong Youn-Kab, Wang Myeong-Hyeon, Lee Wi-Young, Rhee Hae-Ik
Division of Biotechnology, Kangwon National University, Chunchon, Korea.
Nutrition. 2005 Jun;21(6):756-61. doi: 10.1016/j.nut.2004.10.014.
This study investigated the inhibitory effect of pine bark extract (PBE) and needle extract on carbohydrate-hydrolyzing enzymes and the hypoglycemic effect in diabetic mice (Lep(ob) [ob/ob]).
Pine bark and needle were dried and then placed in ethanol, and the extracts were assayed for the measurement of inhibition mode of PBE against alpha-amylase (EC 3.2.1.1) and alpha-glucosidase (EC 3.2.1.20). We also investigated the effect of long-term treatment with extracts on levels of postprandial blood glucose, body weight, food efficiency ratio, and gene expression of glucose transporter-4 in quadriceps muscle in diabetic mice (Lep(ob) [ob/ob]).
The PBE showed competitive inhibition against salivary alpha-amylase and the combination of non-competitive and uncompetitive inhibition against yeast alpha-glucosidase. In animal experiments, PBE effectively suppressed the increase of postprandial blood glucose level by delaying absorption of diet, and body weights of the group that received PBE were significantly lower than that in the group administered 0.5% carboxylmethyl cellulose (control) 21 d after administration.
PBE can be used to suppress postprandial hyperglycemia of diabetic patients. It also can be applied for control of obesity by decreasing the food efficiency ratio, especially carbohydrates.
本研究调查了松树皮提取物（PBE）和松针提取物对碳水化合物水解酶的抑制作用以及对糖尿病小鼠（Lep(ob) [ob/ob]）的降血糖作用。
将松树皮和松针干燥后置于乙醇中，测定提取物对PBE对α-淀粉酶（EC 3.2.1.1）和α-葡萄糖苷酶（EC 3.2.1.20）的抑制模式。我们还研究了提取物长期治疗对糖尿病小鼠（Lep(ob) [ob/ob]）餐后血糖水平、体重、食物效率比以及股四头肌中葡萄糖转运蛋白4基因表达的影响。
PBE对唾液α-淀粉酶表现出竞争性抑制，对酵母α-葡萄糖苷酶表现出非竞争性和反竞争性抑制的组合。在动物实验中，PBE通过延迟饮食吸收有效抑制餐后血糖水平的升高，给药21天后，接受PBE的组的体重显著低于给予0.5%羧甲基纤维素的组（对照组）。
PBE可用于抑制糖尿病患者的餐后高血糖。它还可通过降低食物效率比，特别是碳水化合物的效率比，用于控制肥胖。
