Bio-Medical Laboratory Science and Management (U.G.C. Innovative Funded Department), Vidyasagar University, Midnapore 721 102, West Bengal, India.
J Ethnopharmacol. 2011 Apr 26;135(1):194-6. doi: 10.1016/j.jep.2011.02.034. Epub 2011 Mar 6.
The present experiment was conducted to search out the effect of hydro-methanolic extract of seed of Holarrhena antidysenterica on intestinal α-glucosidase activity in dose dependent manner and on the management of postprandial hyperglycemia in starch loaded rats.
Activity of intestinal α-glucosidase was measured by in vitro method. Fasting blood glucose level was determined by single touch glucometer. Total phenol and flavonoids of seed extract of Holarrhena antidysenterica were estimated using gallic acid and quercetin standard curves, respectively.
The degree of elevation in blood glucose level after starch administration was significantly (p<0.05) less by the extract in respect to the control. The said extract also inhibited α-glucosidase activity having an IC(50) of 0.52mg/ml. Phytochemical study revealed that the extract is rich in phenolic compounds (60.23mg of gallic acid equivalent/g of extract) and flavonoids (360.23mg of quercetin equivalent/g of the extract).
The extract exerts its antihyperglycemic effect by retarding the carbohydrate absorption from intestine through the inhibition in α-glucosidase activity and therefore resists postprandial hyperglycemia.
本实验旨在研究 Holarrhena antidysenterica 种子的水-甲醇提取物对肠道α-葡萄糖苷酶活性的影响，以及对淀粉负荷大鼠餐后高血糖的管理作用。
采用体外法测定肠道α-葡萄糖苷酶的活性。使用单点血糖仪测定空腹血糖水平。使用没食子酸和槲皮素标准曲线分别估算 Holarrhena antidysenterica 种子提取物中的总酚和类黄酮含量。
与对照组相比，提取物显著降低了淀粉给药后血糖水平的升高（p<0.05）。该提取物还抑制了α-葡萄糖苷酶活性，IC（50）为 0.52mg/ml。植物化学研究表明，提取物富含酚类化合物（每克提取物相当于 60.23mg 没食子酸）和类黄酮（每克提取物相当于 360.23mg 槲皮素）。
提取物通过抑制α-葡萄糖苷酶活性来延缓肠道对碳水化合物的吸收，从而发挥抗高血糖作用，因此抵抗餐后高血糖。
