Manzoori Jamshid L, Abdolmohammad-Zadeh Hossein, Amjadi Mohammad
Department of Analytical Chemistry, Faculty of Chemistry, The University of Tabriz, Tabriz, Iran.
Farmaco. 2005 Jun-Jul;60(6-7):575-81. doi: 10.1016/j.farmac.2005.02.003.
The supramolecular interaction of celecoxib (chemically 4-[5-(4-methylphenyl)-3-(trifluoromethyl)-1H-pyrazol-1-yl] benzene sulfonamide) and beta-cyclodextrin (beta-CD) has been studied by spectrofluorimetry. The results showed that beta-CD reacted with celecoxib to form an inclusion complex. 1:1 stoichiometry for beta-CD-celecoxib complex was established and its association constant at different temperatures was calculated by applying a non-linear regression method to the change in the fluorescence of celecoxib that brought about by the presence of beta-CD. The thermodynamic parameters (DeltaH degrees, DeltaS degrees and DeltaG degrees) associated with the inclusion process were also determined. Based on the significant enhancement of the fluorescence intensity of celecoxib produced through complex formation, a simple, rapid and highly sensitive spectrofluorimetric method for the determination of celecoxib in aqueous solution in the presence of beta-CD was developed. The measurement of relative fluorescence intensity was carried out at 390 nm with excitation at 270 nm. A linear relationship between the fluorescence intensity and celecoxib concentration was obtained in the range of 0.1-4.0 microg ml-1, with a correlation coefficient of 0.9996. The detection limit was 7.29 ng ml-1 and the relative standard deviation was 1.28%. The method was successfully applied to the determination of celecoxib in pharmaceutical preparations.
通过荧光光谱法研究了塞来昔布（化学名称为4-[5-(4-甲基苯基)-3-(三氟甲基)-1H-吡唑-1-基]苯磺酰胺）与β-环糊精（β-CD）的超分子相互作用。结果表明，β-CD与塞来昔布反应形成了包合物。确定了β-CD-塞来昔布复合物的化学计量比为1:1，并通过对β-CD存在导致的塞来昔布荧光变化应用非线性回归方法计算了其在不同温度下的缔合常数。还测定了与包合过程相关的热力学参数（ΔH°、ΔS°和ΔG°）。基于通过形成复合物使塞来昔布荧光强度显著增强，建立了一种简单、快速且高度灵敏的荧光光谱法，用于在β-CD存在下测定水溶液中的塞来昔布。在激发波长为270 nm时，于390 nm处测量相对荧光强度。在0.1 - 4.0 μg ml-1范围内，荧光强度与塞来昔布浓度呈线性关系，相关系数为0.9996。检测限为7.29 ng ml-1，相对标准偏差为1.28%。该方法成功应用于药物制剂中塞来昔布的测定。
