Chen Jianjun, Fang Fang, Li Xiangzhong, Chang Haiyan, Chen Ze
College of Life Science, Hunan Normal University, Yuelushan, Changsha, China.
Vaccine. 2005 Jul 29;23(34):4322-8. doi: 10.1016/j.vaccine.2005.03.035.
The ability of a single dose of plasmid DNA encoding neuraminidase (NA) or hemagglutinin (HA) from influenza virus A/PR/8/34 (PR8) (H1N1) to protect against homologous virus infection was examined in BALB/c mice. In the present study, mice were immunized once with 30 microg of NA or HA DNA by electroporation. Four weeks or 28 weeks after immunization, mice were challenged with a lethal dose of homologous virus and the ability of NA or HA DNA to protect the mice from influenza was evaluated. We found that a single inoculation of NA DNA could provide protection against influenza virus challenge as well as long-term protection against viral infection. Whereas, the mice immunized with a single dose of HA DNA could not be protected. In addition, neonatal mice immunized with a single dose of 30 microg of NA DNA could be provided with significant protection against viral infection.
在BALB/c小鼠中检测了单剂量编码甲型流感病毒A/PR/8/34（PR8）（H1N1）神经氨酸酶（NA）或血凝素（HA）的质粒DNA预防同源病毒感染的能力。在本研究中，通过电穿孔用30微克NA或HA DNA对小鼠进行一次免疫。免疫后4周或28周，用致死剂量的同源病毒攻击小鼠，并评估NA或HA DNA保护小鼠免受流感的能力。我们发现，单次接种NA DNA可以提供针对流感病毒攻击的保护以及针对病毒感染的长期保护。然而，用单剂量HA DNA免疫的小鼠无法得到保护。此外，用单剂量30微克NA DNA免疫的新生小鼠可以获得针对病毒感染的显著保护。
