Statz Andrea R, Meagher Robert J, Barron Annelise E, Messersmith Phillip B
Department of Biomedical Engineering, Northwestern University, 2145 Sheridan Road, Evanston, Illinois 60208, USA.
J Am Chem Soc. 2005 Jun 8;127(22):7972-3. doi: 10.1021/ja0522534.
Exposure of therapeutic and diagnostic medical devices to biological fluids is often accompanied by interfacial adsorption of proteins, cells, and microorganisms. Biofouling of surfaces can lead to compromised device performance or increased cost and in some cases may be life-threatening to the patient. Although numerous antifouling polymer coatings have enjoyed short-term success in preventing protein and cell adsorption on surfaces, none have proven ideal for conferring long-term biofouling resistance. Here we describe a new biomimetic antifouling N-substituted glycine polymer (peptoid) containing a C-terminal peptide anchor derived from residues found in mussel adhesive proteins for robust attachment of the polymer onto surfaces. The methoxyethyl side chain of the peptoid portion of the polymer was chosen for its chemical resemblance to the repeat unit of the known antifouling polymer poly(ethylene glycol) (PEG), whereas the composition of the 5-mer anchoring peptide was chosen to directly mimic the DOPA- and Lys-rich sequence of a known mussel adhesive protein. Surfaces modified with this biomimetic peptide-peptoid conjugate exhibited dramatic reduction of serum protein adsorption and resistance to mammalian cell attachment for over 5 months in an in vitro assay. These new synthetic peptide based antifouling polymers may provide long-term control of surface biofouling in the physiologic, marine, and industrial environments.
治疗和诊断医疗设备与生物流体接触时，通常会伴随蛋白质、细胞和微生物在界面的吸附。表面的生物污垢会导致设备性能下降或成本增加，在某些情况下可能会对患者生命构成威胁。尽管许多防污聚合物涂层在防止蛋白质和细胞吸附在表面方面取得了短期成功，但没有一种被证明是赋予长期抗生物污垢的理想材料。在此，我们描述了一种新型仿生防污N-取代甘氨酸聚合物（类肽），它含有一个C端肽锚，该锚源自贻贝粘附蛋白中的残基，用于将聚合物牢固地附着在表面。聚合物类肽部分的甲氧基乙基侧链因其化学结构与已知防污聚合物聚乙二醇（PEG）的重复单元相似而被选用，而五聚体锚定肽的组成则被选择为直接模仿已知贻贝粘附蛋白中富含多巴和赖氨酸的序列。在体外试验中，用这种仿生肽-类肽共轭物修饰的表面在超过5个月的时间里血清蛋白吸附显著减少，并且对哺乳动物细胞附着具有抗性。这些基于新型合成肽的防污聚合物可能会在生理、海洋和工业环境中对表面生物污垢实现长期控制。
