Statz Andrea R, Barron Annelise E, Messersmith Phillip B
Department of Biomedical Engineering, Northwestern University, 2145 Sheridan Rd, Evanston, IL, 60208, USA.
Soft Matter. 2008 Jan 7;4(1):131-139. doi: 10.1039/B711944E.
Peptidomimetic polymers consisting of poly-N-substituted glycine oligomers (polypeptoids) conjugated to biomimetic adhesive polypeptides were investigated as antifouling surface coatings. The polymers were immobilized onto TiO(2) surfaces via an anchoring peptide consisting of alternating residues of 3,4-dihydroxyphenylalanine (DOPA) and lysine. Three polypeptoid side-chain compositions were investigated for antifouling performance and stability toward enzymatic degradation. Ellipsometry and XPS analysis confirmed that purified polymers adsorbed strongly to TiO(2) surfaces, and the immobilized polymers were resistant to enzymatic degradation as demonstrated by mass spectrometry. All polypeptoid-modified surfaces exhibited significant reductions in adsorption of lysozyme, fibrinogen and serum proteins, and were resistant to 3T3 fibroblast cell attachment for up to seven days. Long-term in vitro cell attachment studies conducted for six weeks revealed the importance of polypeptoid side-chain composition, with a methoxyethyl side chain providing superior long-term fouling resistance compared to hydroxyethyl and hydroxypropyl side chains. Finally, attachment of both gram-positive and gram-negative bacteria for up to four days under continuous-flow conditions was significantly reduced on the polypeptoid-modified surfaces compared to unmodified TiO(2) surfaces. The results reveal the influence of polypeptoid side-chain chemistry on short-term and long-term protein, cell and bacterial fouling resistance.
由与仿生粘附多肽共轭的聚-N-取代甘氨酸低聚物（类多肽）组成的拟肽聚合物被研究作为防污表面涂层。这些聚合物通过由3,4-二羟基苯丙氨酸（DOPA）和赖氨酸的交替残基组成的锚定肽固定在TiO(2)表面上。研究了三种类多肽侧链组成的防污性能和对酶降解的稳定性。椭圆偏振光谱法和X射线光电子能谱分析证实，纯化的聚合物强烈吸附在TiO(2)表面上，并且如质谱所示，固定化的聚合物对酶降解具有抗性。所有类多肽修饰的表面在溶菌酶、纤维蛋白原和血清蛋白的吸附方面都有显著降低，并且在长达七天的时间内对3T3成纤维细胞的附着具有抗性。为期六周的长期体外细胞附着研究揭示了类多肽侧链组成的重要性，与羟乙基和羟丙基侧链相比，甲氧基乙基侧链具有更好的长期抗污性能。最后，与未修饰的TiO(2)表面相比，在连续流动条件下，革兰氏阳性和革兰氏阴性细菌在类多肽修饰表面上的附着长达四天显著减少。结果揭示了类多肽侧链化学对短期和长期蛋白质、细胞和细菌抗污性能的影响。
