Zinellu Angelo, Caria Marcello A, Tavera Claudio, Sotgia Salvatore, Chessa Roberto, Deiana Luca, Carru Ciriaco
Chair of Clinical Biochemistry, University of Sassari, Viale San Pietro 43/B, I-07100 Sassari, Italy.
Anal Biochem. 2005 Jul 15;342(2):186-93. doi: 10.1016/j.ab.2005.01.045. Epub 2005 Feb 15.
Traditional clinical assays for nonprotein nitrogen compounds, such as creatine and creatinine, have focused on the use of enzymes or chemical reactions that allow measurement of each analyte separately. Most of these assays are mainly directed to urine quantification, so that their applicability on plasma samples is frequently hard to perform. This work describes a simple free zone capillary electrophoresis method for the simultaneous measurement of creatinine and creatine in human plasma. The effect of analytical parameters such as concentration and pH of Tris-phosphate running buffer and cartridge temperature on resolution, migration times, peak areas, and efficiency was investigated. Good separation was achieved using a 60.2-cm x 75-microm uncoated silica capillary, 75 mmol/L Tris-phosphate buffer, pH 2.25, at 15 degrees C, in less than 8 min. We compared the present method to a validated capillary electrophoresis assay, by measuring plasma creatinine in 120 normal subjects. The obtained data were compared by the Passing-Bablok regression and the Bland-Altman test. Moreover the performance of the developed method was assessed by measuring creatine and creatinine in 16 volunteers prior to and after a moderate physical exercise.
用于检测非蛋白质氮化合物（如肌酸和肌酐）的传统临床检测方法，主要侧重于使用能分别测量每种分析物的酶或化学反应。这些检测方法大多主要针对尿液定量，因此它们在血浆样本上的适用性往往难以实现。本文描述了一种简单的自由区毛细管电泳方法，用于同时测量人血浆中的肌酐和肌酸。研究了诸如磷酸三乙胺运行缓冲液的浓度和pH值以及柱温等分析参数对分离度、迁移时间、峰面积和效率的影响。使用一根60.2厘米×75微米的未涂层石英毛细管、75毫摩尔/升pH值为2.25的磷酸三乙胺缓冲液，在15℃下，不到8分钟即可实现良好的分离。我们通过测量120名正常受试者的血浆肌酐，将本方法与经过验证的毛细管电泳检测方法进行了比较。通过Passing-Bablok回归和Bland-Altman检验对所得数据进行比较。此外，通过测量16名志愿者在适度体育锻炼前后的肌酸和肌酐，评估了所开发方法的性能。
