Alonso Rodrigo, Mateo Estibaliz, Churruca Estibaliz, Martinez Irati, Girbau Cecilia, Fernández-Astorga Aurora
Departamento de Inmunología, Microbiología y Parasitología, Facultad de Farmacia, Universidad del País Vasco (UPV-EHU), Paseo de la Universidad 7, 01006 Vitoria-Gasteiz, Spain.
J Microbiol Methods. 2005 Oct;63(1):99-103. doi: 10.1016/j.mimet.2005.03.013.
Twenty Campylobacter jejuni and 16 Campylobacter coli strains isolated from humans and food/animals, including 17 isolates resistant to erythromycin, were analyzed. A combined mismatch amplification mutation assay-PCR technique was developed to detect the mutations A 2074 C and A 2075 G in the 23S rRNA gene associated with erythromycin resistance. All high-level erythromycin-resistant strains examined by DNA sequencing carried the transition mutation A 2075 G, whereas no isolate carried the A 2074 C mutation. No mutations were found among the susceptible and low-level erythromycin-resistant strains.
对从人类和食品/动物中分离出的20株空肠弯曲菌和16株结肠弯曲菌菌株进行了分析，其中包括17株对红霉素耐药的分离株。开发了一种错配扩增突变检测-PCR联合技术，以检测与红霉素耐药相关的23S rRNA基因中的A 2074 C和A 2075 G突变。通过DNA测序检测的所有高水平红霉素耐药菌株均携带A 2075 G转换突变，而没有分离株携带A 2074 C突变。在敏感和低水平红霉素耐药菌株中未发现突变。
