通过聚合酶链反应（PCR）和线性探针分析快速检测空肠弯曲菌/结肠弯曲菌中与红霉素耐药相关的突变
Rapid detection of mutations associated with resistance to erythromycin in Campylobacter jejuni/coli by PCR and line probe assay.
作者信息
Niwa H, Chuma T, Okamoto K, Itoh K
机构信息
Laboratory of Veterinary Public Health, Graduate School of Agricultural and Life Sciences, the University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
出版信息
Int J Antimicrob Agents. 2001 Oct;18(4):359-64. doi: 10.1016/s0924-8579(01)00425-3.
Mutation of 23S rDNA is one of the mechanisms of erythromycin resistance. PCR and line probe assay (PCR-LiPA) with ten oligonucleotide probes were developed to detect the mutations associated with macrolide resistance at positions of 2072, 2073 and 2074 in 23S rDNA of Campylobacter jejuni/coli. A2074-->G mutation was detected in 12 of 25 isolates, which were resistant to erythromycin. No other mutations in 23S rDNA were detected. The rest of the strains were susceptible to erythromycin and no mutation in 23S rDNA was detected. Six laboratory induced erythromycin resistant mutants had no mutations in 23S rDNA. PCR-LiPA is a useful and rapid method to detect mutations in 23S rDNA associated with erythromycin resistance in C. jejuni/coli.
23S rDNA突变是红霉素耐药的机制之一。开发了一种PCR和线性探针分析（PCR-LiPA）方法，使用十个寡核苷酸探针来检测空肠弯曲菌/结肠弯曲菌23S rDNA中2072、2073和2074位点与大环内酯耐药相关的突变。在25株对红霉素耐药的分离株中，有12株检测到A2074→G突变。未检测到23S rDNA的其他突变。其余菌株对红霉素敏感，未检测到23S rDNA突变。六个实验室诱导的红霉素耐药突变体在23S rDNA中没有突变。PCR-LiPA是一种检测空肠弯曲菌/结肠弯曲菌中与红霉素耐药相关的23S rDNA突变的有用且快速的方法。
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