Majima Tokifumi, Funakosi Tadanao, Iwasaki Norimasa, Yamane Shin-Tarou, Harada Kazuo, Nonaka Sachiko, Minami Akio, Nishimura Shin-Ichiro
Department of Orthopaedic Surgery, Hokkaido University School of Medicine, Kita 15, Nishi 7, Kita-Ku, Sapporo, 060-8638, Japan.
J Orthop Sci. 2005 May;10(3):302-7. doi: 10.1007/s00776-005-0891-y.
Selecting the material for a scaffold is critically important for the success of tissue engineering. To simplify complicated biosynthetic matrices and achieve a novel class of potential materials, a model of polyion complex fibers was prepared from alginate and chitosan. In the current in vitro study, we thought that alginate-based chitosan hybrid biomaterials could provide excellent supports for fibroblast adhesion. In the current study, alginate polymer fiber (alginate group) and alginate-based chitosan hybrid polymer fibers (alginate with 0.05% chitosan, alginate-chitosan 0.05% group; alginate with 0.1% chitosan, alginate-chitosan 0.1% group) were originally prepared. We investigated the adhesion behavior of rabbit tendon fibroblast onto alginate polymer fibers versus the adhesion of the fibroblast onto alginate-based chitosan hybrid polymer fibers. Furthermore, mechanical properties and synthesis of the extracellular matrix were investigated. Mechanically, the novel fiber has considerable tensile strength of more than 200 MPa. We demonstrated that the alginate-based chitosan hybrid polymer fibers showed much improved adhesion capacity with fibroblast compared with alginate polymer fiber. Additionally, morphologic studies revealed the dense fiber of the type I collagen produced by the fibroblast in the hybrid polymer fibers. We concluded that an alginate-based chitosan hybrid polymer fiber has considerable potential as a desirable biomaterial scaffold for tendon and ligament tissue engineering.
选择用于支架的材料对于组织工程的成功至关重要。为了简化复杂的生物合成基质并获得一类新型潜在材料，由海藻酸盐和壳聚糖制备了聚离子复合纤维模型。在当前的体外研究中，我们认为基于海藻酸盐的壳聚糖混合生物材料可为成纤维细胞粘附提供良好的支持。在本研究中，最初制备了海藻酸盐聚合物纤维（海藻酸盐组）和基于海藻酸盐的壳聚糖混合聚合物纤维（含0.05%壳聚糖的海藻酸盐，海藻酸盐-壳聚糖0.05%组；含0.1%壳聚糖的海藻酸盐，海藻酸盐-壳聚糖0.1%组）。我们研究了兔肌腱成纤维细胞在海藻酸盐聚合物纤维上的粘附行为与成纤维细胞在基于海藻酸盐的壳聚糖混合聚合物纤维上的粘附情况。此外，还研究了细胞外基质的力学性能和合成。在力学方面，这种新型纤维具有超过200MPa的可观拉伸强度。我们证明，与海藻酸盐聚合物纤维相比，基于海藻酸盐的壳聚糖混合聚合物纤维对成纤维细胞的粘附能力有很大提高。此外，形态学研究揭示了在混合聚合物纤维中由成纤维细胞产生的I型胶原致密纤维。我们得出结论，基于海藻酸盐的壳聚糖混合聚合物纤维作为用于肌腱和韧带组织工程的理想生物材料支架具有相当大的潜力。
