Rahul S, Kataria J M, Senthilkumar N, Dhama K, Sylvester S A, Uma R
Division of Avian Diseases, Indian Veterinary Research Institute, Izatnagar--243122 (U.P.), India.
Acta Virol. 2005;49(2):139-43.
Eight fowl adenovirus (FAdV) isolates obtained from different geographical regions of India were typed by a virus-neutralization test (VNT) using rabbit antisera against all the 12 serotypes of FAdV and by PCR for the hyper-variable region of hexon gene combined with restriction fragment length polymorphism (RFLP) analysis using AluI and MboI restriction enzymes. It was found that six isolates belonged to FAdV-4, one to FAdV-12 and one to both of them. This study revealed the involvement of FAdV-12 alone or in association with FAdV-4 in precipitating inclusion body hepatitis--hydropericardium syndrome (IBH-HPS) among poultry flocks in the country.
从印度不同地理区域获得的8株禽腺病毒（FAdV）分离株，通过使用针对所有12种FAdV血清型的兔抗血清进行病毒中和试验（VNT）进行分型，并通过针对六邻体基因高变区的PCR结合使用AluI和MboI限制性内切酶的限制性片段长度多态性（RFLP）分析进行分型。结果发现，6株分离株属于FAdV-4，1株属于FAdV-12，1株同时属于这两种血清型。本研究揭示了FAdV-12单独或与FAdV-4联合参与该国禽类群体中包涵体肝炎-心包积水综合征（IBH-HPS）的发生。
