Yang Hye-Jin, Lee Sang-Hyun, Jin Yong, Choi Jin-Hyang, Han Chang-Hoon, Lee Mun-Han
Department of Biochemistry, College of Veterinary Medicine, Seoul National University, Seoul, Korea.
J Vet Sci. 2005 Jun;6(2):103-9.
The toxicity of acrylamide was evaluated through mutagenicity of Salmonella, chromosome aberration of Chinese hamster lung fibroblasts, micronucleus formation in mice and reproductive toxicity in rats. Based on Ames test, acrylamide showed mutagenic potency for strains TA98 and TA100. Moreover, both chromosomal aberration assay and micronucleus assay indicated that acrylamide might have genotoxic potency; the chromosomal aberration frequencies were observed to be proportional to acrylamide concentrations of 5-50 mM, and acrylamide significantly increased micronuclei in peripheral blood cells of mice at doses of higher than 72.5 mg/kg. Male rats were treated with acrylamide at doses of 0, 5, 15, 30, 45, or 60 mg/kg/day for 5 consecutive days, and the toxicity of acrylamide was observed. In the group treated with the highest dose of acrylamide (60 mg/kg/day), the loss of body weight and reduced testis weight were observed. Also the epididymides weights were reduced significantly in all the groups treated with acrylamide. The number of sperms in cauda epididymidis decreased significantly in an acrylamide dose-dependent manner. Rats treated with 60 mg/kg/day of acrylamide showed several histopathological lesions in the seminiferous tubules. There were thickening and multiple layering of the tubular endothelium, and the formation of many multinucleated giant cells in seminiferous tubules. Taken together, acrylamide not only causes the genotoxicity of eukaryotic cells and mice but also shows the toxicological effects on reproductive system in male rats.
通过沙门氏菌的致突变性、中国仓鼠肺成纤维细胞的染色体畸变、小鼠的微核形成以及大鼠的生殖毒性来评估丙烯酰胺的毒性。基于艾姆斯试验，丙烯酰胺对TA98和TA100菌株显示出致突变能力。此外，染色体畸变试验和微核试验均表明丙烯酰胺可能具有遗传毒性；观察到染色体畸变频率与5-50 mM的丙烯酰胺浓度成正比，并且丙烯酰胺在高于72.5 mg/kg的剂量下显著增加了小鼠外周血细胞中的微核。雄性大鼠连续5天接受0、5、15、30、45或60 mg/kg/天剂量的丙烯酰胺处理，并观察丙烯酰胺的毒性。在接受最高剂量丙烯酰胺（60 mg/kg/天）处理的组中，观察到体重减轻和睾丸重量降低。在用丙烯酰胺处理的所有组中，附睾重量也显著降低。附睾尾部的精子数量以丙烯酰胺剂量依赖性方式显著减少。用60 mg/kg/天的丙烯酰胺处理的大鼠在生精小管中出现了几种组织病理学损伤。存在小管内皮增厚和多层化，并且在生精小管中形成了许多多核巨细胞。综上所述，丙烯酰胺不仅会导致真核细胞和小鼠的遗传毒性，还会对雄性大鼠的生殖系统产生毒理学影响。
