Oem Jae Ku, Lee Kwang Nyeong, Cho In Soo, Kye Soo Jeong, Park Jee Yong, Park Jong Hyeon, Kim Yong Joo, Joo Yi Seok, Song Hee Jong
National Veterinary Research and Quarantine Service, Ministry of Agriculture and Forestry, Anyang, Korea.
J Vet Sci. 2005 Jun;6(2):117-24.
From May to June 2002, a total of 16 foot-and mouth disease (FMD) outbreaks due to the serotype O virus, Pan Asia strain, were recorded in Korea. The viruses were identified by antigen ELISA, RT-PCR and sequence analysis. The overall nucleotide sequence divergence of the VP1 region among the 4 isolates in 2002 was 0 to 1.4%, but between O/SKR/2002 and O/SKR/2000 isolates was 1.9-4.9%. Phylogenetic analysis with the some known strains from East Asian countries showed that the 4 Korean isolates in 2002 formed one distinct cluster, which different from clusters of Korean isolates in 2000, with in the same lineage of the ME-SA topotype strains. Deduced amino acid sequences around neutralizable antigenic site on VP1 site of O/SKR/2002 isolates were aligned and compared with other strains. At the antigenic site 1, the replacements of the critical amino acid residues at position 144 from V to L and at position 152 from A to T were observed in O/SKR/2002 viruses. For antigenic site 2 and 4, there were not significant variations in general. At the antigenic site 3, the substitutions of amino acid residues were present at positions 54 and 56 in O/SKR/2002 isolates and an alternative residue I at position 54 are observed only at the sequence of O/SKR/AS/2002 (cow) virus. And the substitution (L-->P) of significant residue at position 144 was detected at the amino acid sequence of the O/SKR/2002 (cow) virus.
2002年5月至6月期间，韩国共记录到16起因O型病毒泛亚株引发的口蹄疫疫情。通过抗原酶联免疫吸附测定、逆转录聚合酶链反应及序列分析对病毒进行了鉴定。2002年4株分离病毒VP1区的总体核苷酸序列差异为0至1.4%，但O/SKR/2002与O/SKR/2000分离株之间的差异为1.9 - 4.9%。对来自东亚国家的一些已知毒株进行系统发育分析表明，2002年的4株韩国分离病毒形成了一个独特的簇，与2000年的韩国分离株簇不同，属于中东-南非（ME-SA）拓扑型毒株的同一谱系。对O/SKR/2002分离株VP1位点上可中和抗原位点周围的推导氨基酸序列进行比对，并与其他毒株进行比较。在抗原位点1，O/SKR/2002病毒中观察到第144位关键氨基酸残基从缬氨酸（V）替换为亮氨酸（L），以及第152位从丙氨酸（A）替换为苏氨酸（T）。对于抗原位点2和4，总体上没有显著变异。在抗原位点3，O/SKR/2002分离株在第54和56位存在氨基酸残基替换，仅在O/SKR/AS/2002（牛）病毒序列中观察到第54位的替代残基异亮氨酸（I）。并且在O/SKR/2002（牛）病毒的氨基酸序列中检测到第144位显著残基的替换（亮氨酸（L）变为脯氨酸（P））。
