König G A, Palma E L, Maradei E, Piccone M E
Instituto de Biotecnología, CICVyA, Instituto Nacional de Tecnología Agropecuaria, CC25, Castelar 1712, Buenos Aires, Argentina.
Vet Microbiol. 2007 Sep 20;124(1-2):1-15. doi: 10.1016/j.vetmic.2007.03.015. Epub 2007 Mar 27.
During 2000-2002 a foot-and-mouth disease (FMD) epizootic affected Argentina and spread across the country resulting in more than 2500 outbreaks. In order to study the evolution of the FMD viruses (FMDV) and help with disease control measures, a genetic characterization and phylogenetic analysis was performed of 43 field isolates representative of the epizootic. The nucleotide sequence of the VP1-coding region was determined for the viruses and used in this study. Two serotype A lineages, A/Arg/00 and A/Arg/01 (1000/1000 bootstrap value) and two different serotype O/Arg/00 lineages (848/1000 bootstrap value) were identified. Phylogenetic analysis showed that viruses A/Arg/01 and O/Arg/00 could be related with former South American isolates, while the origin of A Argentina 2000 viruses remains unclear. Comparison of the amino acid sequences with vaccine reference strains revealed differences at critical antigenic sites for emergent strains A/Arg/00 and A/Arg/01, leading to a change in the current vaccine formulation.
2000年至2002年期间，一场口蹄疫疫情影响了阿根廷并蔓延至全国，导致2500多处疫情爆发。为了研究口蹄疫病毒（FMDV）的演变并协助制定疾病控制措施，对代表该疫情的43株野外分离株进行了基因特征分析和系统发育分析。本研究测定了这些病毒VP1编码区的核苷酸序列。鉴定出两个A型血清型谱系，即A/Arg/00和A/Arg/01（自展值为1000/1000），以及两个不同的O/Arg/00型血清型谱系（自展值为848/1000）。系统发育分析表明，A/Arg/01和O/Arg/00病毒可能与以前的南美分离株有关，而2000年阿根廷A型病毒的起源仍不清楚。将氨基酸序列与疫苗参考毒株进行比较后发现，新出现的A/Arg/00和A/Arg/01毒株在关键抗原位点存在差异，这导致了当前疫苗配方的改变。
