Rizk Mohamed Abdo, Mahmoud Motamed Elsayed, El-Sayed Shimaa Abd El-Salam, Salman Doaa
Department of Internal Medicine and Infectious Diseases, Faculty of Veterinary Medicine, Mansoura University, Mansoura, 35516, Egypt.
Department of Animal Behavior and Husbandry, Faculty of Veterinary Medicine, Sohag University, Sohag, 82524, Egypt.
Trop Anim Health Prod. 2017 Dec;49(8):1723-1731. doi: 10.1007/s11250-017-1383-8. Epub 2017 Aug 22.
In the current study, we compared the therapeutic effects of a non-steroidal and a steroidal anti-inflammatory drug on the production of pro-inflammatory cytokines, interleukin-1β (IL-1β), interleukin-6 (IL-6), interleukin-12p40 (IL-12p40), interferon gamma (IFNγ), and tumor necrosis factor alpha (TNF-α) in the blood of water buffalo (Bubalus bubalis) calves naturally infected by bronchopneumonia. Twenty-seven buffalo calves (7 ± 2-month-old, 163 ± 12 kg) reared in smallholder farms in El-Dakahlia province in Egypt were identified to have bronchopneumonia and randomly allocated into three equal groups. Ten clinically healthy buffalo calves with negative bronchoalveolar lavage results were served as negative control. Diseased calves were treated with tulathromycin alone, a combination of tulathromycin with dexamethasone (steroidal anti-inflammatory drug) or tulathromycin with flunixin meglumine (non-steroidal anti-inflammatory drug). The results revealed significant elevations (P < 0.05) in the production of selected cytokines in all diseased calves in comparison with healthy animals. Six days post-treatment, a significant inhibition (P < 0.05) in the production of all assessed cytokines was observed in the blood of all treated calves. Interestingly, the serum concentrations of IL-1β and IL-12p40 were returned to the normal levels in pneumonic calves treated with the combination therapy of tulathromycin and flunixin meglumine. A strong significant positive correlation (P < 0.05) was detected between clinical sum scoring and IL-12p40 and TNF-α concentrations. The obtained results indicate the selectively potent anti-inflammatory effect of flunixin meglumine on the production of pro-inflammatory cytokines in pneumonic buffalo calves and highlight the efficacy of flunixin meglumine in the treatment of bronchopneumonia in buffalo calves when used in combination with tulathromycin.
在本研究中，我们比较了一种非甾体类抗炎药和一种甾体类抗炎药对自然感染支气管肺炎的水牛（Bubalus bubalis）犊牛血液中促炎细胞因子白细胞介素-1β（IL-1β）、白细胞介素-6（IL-6）、白细胞介素-12p40（IL-12p40）、干扰素γ（IFNγ）和肿瘤坏死因子α（TNF-α）产生的治疗效果。在埃及达卡利亚省的小农场上饲养的27头水牛犊牛（7±2月龄，163±12千克）被确诊患有支气管肺炎，并随机分为三组。十头支气管肺泡灌洗结果为阴性的临床健康水牛犊牛作为阴性对照。患病犊牛分别接受单独的图拉霉素治疗、图拉霉素与地塞米松（甾体类抗炎药）联合治疗或图拉霉素与氟尼辛葡甲胺（非甾体类抗炎药）联合治疗。结果显示，与健康动物相比，所有患病犊牛中所选细胞因子的产生均显著升高（P<0.05）。治疗后六天，在所有接受治疗的犊牛血液中观察到所有评估细胞因子的产生均有显著抑制（P<0.05）。有趣的是，用图拉霉素和氟尼辛葡甲胺联合治疗的肺炎犊牛中，IL-1β和IL-12p40的血清浓度恢复到了正常水平。临床综合评分与IL-12p40和TNF-α浓度之间检测到强显著正相关（P<0.05）。所得结果表明氟尼辛葡甲胺对肺炎水牛犊牛促炎细胞因子的产生具有选择性强效抗炎作用，并突出了氟尼辛葡甲胺与图拉霉素联合使用时在治疗水牛犊牛支气管肺炎方面的疗效。
