Guo Ruihua, Zhai Li, Liu Zhengmeng, Wang Heping, Zhao Yunsen, Sun Yanmei, Zou Hongbo
North China Coal Medical College, Tangshan 063000.
Zhong Yao Cai. 2005 Jan;28(1):38-40.
To study the effects of lobster sauce and polysaccharide extracts on alpha-1,4-glucosidase activity by establishing on enzyme-inhibitor model.
Glucosidase activity was assayed at PH optima by PNPG substrate. Lobster sauce extracts were prepared by soaking, centrifagation. Polysaccharide were prepared by soaking, centrifagation, separation.
Lobster sauce and polysaccharide can inhibit alpha-1,4-glucosidase.
This finding suggested that their hypoglycemic mechanism was similar to Acarbose. Lobster sauce may contain phenyalanine.
通过建立酶-抑制剂模型，研究豆豉及其多糖提取物对α-1,4-葡萄糖苷酶活性的影响。
以对硝基苯-α-D-吡喃葡萄糖苷（PNPG）为底物，在最适pH条件下测定糖苷酶活性。豆豉提取物通过浸泡、离心制备。多糖通过浸泡、离心、分离制备。
豆豉及其多糖能抑制α-1,4-葡萄糖苷酶。
该发现表明它们的降血糖机制与阿卡波糖相似。豆豉可能含有苯丙氨酸。
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