Zhang Hai-Feng, Dong Ya-Lin, Hu Sa-Sa, Ren Shao-Jun
Department of Pharmacy, The First Affiliated Hospital of Medical School, Xi'an Jiaotong University, Xi'an 710061, China.
Zhong Yao Cai. 2008 Jul;31(7):1024-7.
To compare alpha-glycosidase inhibitor microamount screening models which are established by two different resources alpha-glycosidase to screen the Chinese herbal medicines which have great alpha-glycosidase inhibition.
Comparing the activities of two different resources alpha-glycosidase with glucose oxidase method, then establishing optimal reaction conditions. Extracting the water soluble compositions of Chinese gallnut, paeoniae radix, Glycyrrhiza, rhubarb, fructus by boiling ,then using the alpha-glycosidase inhibition model to check and compare their alpha-glycosidase inhibitions.
Five Chinese herbal medicines all had alpha-glycosidase inhibition, and the greatest was Chinese gallnut.
The activities of two resources alpha-glycosidase are certainly different, but they do not have significant influence on findings of screening alpha-glycosidase inhibitors in vitro. Water soluble compositions of five Chinese herbal medicines have alpha-glycosidase inhibition.
比较由两种不同来源的α-糖苷酶建立的α-糖苷酶微量筛选模型，以筛选出具有较强α-糖苷酶抑制作用的中药。
用葡萄糖氧化酶法比较两种不同来源α-糖苷酶的活性，进而建立最佳反应条件。采用水煮法提取五倍子、白芍、甘草、大黄、果实的水溶性成分，然后用α-糖苷酶抑制模型检测并比较它们对α-糖苷酶的抑制作用。
五种中药均有α-糖苷酶抑制作用，其中五倍子的抑制作用最强。
两种来源的α-糖苷酶活性确实存在差异，但对体外筛选α-糖苷酶抑制剂的结果没有显著影响。五种中药的水溶性成分具有α-糖苷酶抑制作用。
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