Lin Xiaoping, He Shaoheng, Xie Hua
Department of Allergy, the PLA Center of Respiratory and Allergic Disease Diagnosis Management, General Hospital of Shenyang Military Region, Shenyang, 110016, China.
Lin Chuang Er Bi Yan Hou Ke Za Zhi. 2005 Mar;19(5):195-7.
To investigate the correlations between plasma levels of IL-18, IL-4, IFN-gamma, IgE and eosinophil cationic protein (ECP) in the patients with seasonal allergic rhinitis (SAR), and their clinical implications.
A total of 89 patients with SAR were divided into two groups, acute group and remission group. Thirty healthy controls were recruited into the study. The levels of IL-18, IL-4, IFN-gamma were detected with sandwich ELISA procedures and sIgE (specific IgE), tIgE (total IgE) and ECP levels were measured with fluorescent enzyme immunoassay using UniCAP system in each group.
(1) At acute stage of patients with SAR, the plasma levels of tIgE and ECP were significantly higher than that of the patients at remission stage and healthy controls (P < 0.01, respectively). There was not statistical difference between acute and remission stage (P > 0.05) in the levels of sIgE, but it was significantly higher than that of healthy controls (P < 0.01). (2) At acute stage of patients with SAR, plasma levels of IL-18 and IL-4 were significantly higher than those of the patients at remission stage and healthy controls (P < 0.01, respectively), the levels of IL-18 at remission stage was still higher than that of healthy controls (P < 0.01). (3) There were positive correlations between the plasma levels of IL-18 and IL-4, tIgE, ECP, respectively in acute group, whereas there were negative correlations between the IL-4, tIgE and IFN-gamma, respectively. There were no correlations between levels of IL-18 and IFN-gamma, sIgE.
The plasma levels of IL-18, tIgE, IL-4 and ECP were significantly higher in the patients with SAR, and there were positive correlations between them respectively. It was suggested that IL-18 is likely to be involved in the pathogenesis of allergic inflammation.
探讨季节性变应性鼻炎（SAR）患者血浆白细胞介素-18（IL-18）、白细胞介素-4（IL-4）、γ干扰素（IFN-γ）、免疫球蛋白E（IgE）及嗜酸性粒细胞阳离子蛋白（ECP）水平之间的相关性及其临床意义。
将89例SAR患者分为急性组和缓解组。招募30名健康对照者纳入研究。采用夹心酶联免疫吸附测定法检测各组IL-18、IL-4、IFN-γ水平，采用荧光酶免疫测定法使用UniCAP系统测定特异性IgE（sIgE）、总IgE（tIgE）和ECP水平。
（1）SAR患者急性期血浆tIgE和ECP水平显著高于缓解期患者和健康对照者（分别为P<0.01）。sIgE水平在急性期与缓解期之间无统计学差异（P>0.05），但显著高于健康对照者（P<0.01）。（2）SAR患者急性期血浆IL-18和IL-4水平显著高于缓解期患者和健康对照者（分别为P<0.01），缓解期IL-18水平仍高于健康对照者（P<0.01）。（3）急性组血浆IL-18水平分别与IL-4、tIgE、ECP呈正相关，而IL-4、tIgE分别与IFN-γ呈负相关。IL-18水平与IFN-γ、sIgE无相关性。
SAR患者血浆IL-18、tIgE、IL-4和ECP水平显著升高，且它们之间分别呈正相关。提示IL-18可能参与变应性炎症的发病机制。
