Fan Chun-hong, Yang Tie-sheng, Tang Su-mei, Wang Qin, Zhang Zheng
Department of Clinical Laboratory, Peking University People's Hospital, Beijing 100044, China.
Zhonghua Yi Xue Za Zhi. 2008 Dec 30;88(48):3422-4.
To analyze the diagnostic significance of serum total IgE, specific IgE (SIgE), Phadiatop, and eosinophil cationic protein (ECP) in allergic rhinitis and bronchial asthma.
The serum total IgE, SIgE , and Phadiatop were tested in 122 patients with allergic rhinitis. The ECP, SIgE, and Phadiatop were tested in 135 patients with bronchial asthma. Forty healthy persons were used as controls. The serum total IgE were tested by electrochemiluminescence. The serum Phadiatop, ECP, and SIgE were tested by fluorescent-enzyme linked immunosorbent assay.
The serum total IgE positive rate of the 122 allergic rhinitis patients was 37.7%, significantly higher than that of the healthy controls (2.5%, chi2 = 18.13, P < 0.01). The specificity of total IgE of the allergic rhinitis patients were 97.5%. The serum ECP positive rate of the bronchial asthma patients was 65.9%, significantly higher than that of the healthy controls (20.0%, chi2 = 26.34, P < 0.01). The sensitivity and specificity of the bronchial asthma patients were 65.9% and 80.0%. The Phadiatop positive rates of the allergic rhinitis and the bronchial asthma patients were 50.0% and 31.9% respectively, both significantly higher than that of the control (0, chi2 = 32.08, 16.89, both P < 0.01). Most kinds of SIgE were from the allergens, such as dust mite, dust, and Artemisia etc.
Increase of serum ECP level is an important indicator of airway inflammation activity and inflammation serious degree in the bronchial asthma patients. The increase of serum total IgE has an important diagnostic significance in allergic rhinitis. It is important to detect SIgE and Phadiatop in diagnosis and monitoring of allergic rhinitis and bronchial asthma.
分析血清总IgE、特异性IgE（SIgE）、吸入性变应原过筛试验（Phadiatop）及嗜酸性粒细胞阳离子蛋白（ECP）在变应性鼻炎和支气管哮喘中的诊断意义。
对122例变应性鼻炎患者检测血清总IgE、SIgE及Phadiatop；对135例支气管哮喘患者检测ECP、SIgE及Phadiatop。选取40例健康人作为对照。血清总IgE采用电化学发光法检测；血清Phadiatop、ECP及SIgE采用荧光酶联免疫吸附试验检测。
122例变应性鼻炎患者血清总IgE阳性率为37.7%，显著高于健康对照组（2.5%，χ2 = 18.13，P < 0.01）。变应性鼻炎患者总IgE的特异性为97.5%。支气管哮喘患者血清ECP阳性率为65.9%，显著高于健康对照组（20.0%，χ2 = 26.34，P < 0.01）。支气管哮喘患者的敏感性和特异性分别为65.9%和80.0%。变应性鼻炎和支气管哮喘患者的Phadiatop阳性率分别为50.0%和31.9%，均显著高于对照组（0，χ2 = 32.08、16.89，均P < 0.01）。大多数种类的SIgE来自变应原，如尘螨、灰尘及蒿草等。
血清ECP水平升高是支气管哮喘患者气道炎症活动及炎症严重程度的重要指标。血清总IgE升高在变应性鼻炎中有重要诊断意义。检测SIgE和Phadiatop对变应性鼻炎和支气管哮喘的诊断及病情监测具有重要意义。
