Picker Susanne M, Steisel Alexander, Gathof Birgit S
Transfusion Medicine, University of Cologne, Cologne, Germany.
Transfusion. 2008 Aug;48(8):1685-92. doi: 10.1111/j.1537-2995.2008.01778.x. Epub 2008 May 29.
The aim of this study was to examine the effects of a new riboflavin-based pathogen reduction technology (PRT), the Mirasol PRT process (Navigant Biotechnologies) on platelet (PLT) storage lesion development.
A three-arm in vitro study was conducted comparing cell quality of apheresis PLTs (n = 12 each) treated with Mirasol PRT (M) to untreated (C) and gamma-irradiated units (X) collected from the same donors and stored for up to 7 days under equal conditions.
PLT count, lactate dehydrogenase, and K+ release of M units were not significantly different from C units, indicating retention of cell integrity during storage. The immediate effect (Day 1) of PRT treatment was a significant decrease in hypotonic shock response (M, 80.6 +/- 7.8% vs. 89.2 +/- 8.3%) and aggregation (M, 85.7 +/- 15.2%/min vs. 111.8 +/- 31.5%/min) as well as a significant acceleration of mitochondrial membrane depolarization (M, 1.43 +/- 0.44% vs. 0.91 +/- 0.27%) and P-selectin expression (M, 38.4 +/- 13.8% vs. 15.8 +/- 7.7%) resulting in lower swirl scores on Day 5 (1.5 +/- 0.7 vs. 2.7 +/- 0.4). Significantly higher glucose consumption (60 +/- 13 nmol/10(12) cells/hr vs. 31 +/- 9 nmol/10(12) cells/hr) and lactate production rates (82 +/- 17 nmol/10(12) cells/hr vs. 40 +/- 8 nmol/10(12) cells/hr) caused higher acidity in treated units (pH on Day 5, 6.97 +/- 0.15 vs. 7.42 +/- 0.10). After PRT treatment, oxidative metabolism was still active and, from calculation of oxygen consumption (1.09 +/- 0.23 nmol/min/10(9) PLTs), appeared to be up regulated relative to controls (0.76 +/- 0.27 nmol/min/10(9) PLTs).
Although storage variables clearly showed the effects of PRT treatment, apheresis PLTs treated with Mirasol PRT retained cell quality during 5 days of storage without loss of mitochondria-based oxidative respiration.
本研究旨在探讨一种基于核黄素的新型病原体灭活技术（PRT），即Mirasol PRT工艺（Navigant生物技术公司）对血小板（PLT）储存损伤发展的影响。
进行了一项三臂体外研究，比较经Mirasol PRT处理的单采血小板（每组n = 12）（M组）与未经处理的（C组）以及来自相同供体的γ射线辐照血小板单位（X组）的细胞质量，所有样本在相同条件下储存长达7天。
M组血小板计数、乳酸脱氢酶和钾离子释放与C组无显著差异，表明储存期间细胞完整性得以保留。PRT处理的即时效应（第1天）是低渗休克反应显著降低（M组为80.6 +/- 7.8%，C组为89.2 +/- 8.3%）和聚集显著降低（M组为85.7 +/- 15.2%/分钟，C组为111.8 +/- 31.5%/分钟），以及线粒体膜去极化显著加速（M组为1.43 +/- 0.44%，C组为0.91 +/- 0.27%）和P - 选择素表达显著增加（M组为38.4 +/- 13.8%，C组为15.8 +/- 7.7%），导致第5天的涡旋评分降低（分别为1.5 +/- 0.7和2.7 +/- 0.4）。处理组葡萄糖消耗（60 +/- 13 nmol/10¹²细胞/小时对31 +/- 9 nmol/10¹²细胞/小时）和乳酸生成率显著更高（82 +/- 17 nmol/10¹²细胞/小时对40 +/- 8 nmol/10¹²细胞/小时），导致处理组酸度更高（第5天pH值为6.97 +/- 0.15，C组为7.42 +/- 0.10）。PRT处理后，氧化代谢仍然活跃，根据氧气消耗计算（1.09 +/- 0.23 nmol/分钟/10⁹个血小板），相对于对照组（0.76 +/- 0.27 nmol/分钟/10⁹个血小板）似乎上调。
尽管储存变量清楚地显示了PRT处理的效果，但经Mirasol PRT处理的单采血小板在储存5天期间保持了细胞质量，且基于线粒体的氧化呼吸未丧失。
