Lin Xiao, Xu De-Sheng, Feng Yi, Shen Lan
Department of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
Anal Biochem. 2005 Jul 15;342(2):179-85. doi: 10.1016/j.ab.2005.04.025.
Interest in antimyocardial ischemic activity of a graminan-type fructosan with an average molecular weight of 5,000 Da from Ophiopogon japonicus (FOJ-5) has necessitated the development of a sensitive and specific method to study its pharmacokinetics. An HPLC method with modified postcolumn fluorescence derivatization to determine FOJ-5 in plasma was developed in this study. The Shodex Sugar KS-802 high-performance gel column was chosen for separating FOJ-5 from its degradation products and endogenous carbohydrates. The postcolumn procedure involved acid hydrolysis of the column eluate at 150 degrees C, which decreased the detection limit for FOJ-5 from 1 microg to 25 ng, followed by fluorometric reaction with guanidine in an alkaline medium at 90 degrees C. The clearance of FOJ-5 from the bodies of rats following intravenous injection displayed a complex type of kinetics involving at least two compartments, and the half-life of the elimination of FOJ-5 from plasma administered at 15 mg/kg (18.1 min) was quicker than that administered at 50mg/kg (28.9 min). This modified approach can also be used for microanalysis of both nonreducing oligosaccharides and other neutral polysaccharides.
对来源于麦冬的平均分子量为5000道尔顿的禾本科型果聚糖（FOJ - 5）的抗心肌缺血活性的研究兴趣，使得开发一种灵敏且特异的方法来研究其药代动力学成为必要。本研究建立了一种采用改进的柱后荧光衍生化的高效液相色谱法来测定血浆中的FOJ - 5。选用Shodex Sugar KS - 802高效凝胶柱将FOJ - 5与其降解产物及内源性碳水化合物分离。柱后程序包括在150℃对柱洗脱液进行酸水解，这将FOJ - 5的检测限从1微克降低至25纳克，随后在90℃的碱性介质中与胍进行荧光反应。静脉注射后，FOJ - 5在大鼠体内的清除呈现出一种复杂的动力学类型，涉及至少两个房室，且以15毫克/千克给药时血浆中FOJ - 5消除的半衰期（18.1分钟）比以50毫克/千克给药时（28.9分钟）更快。这种改进方法也可用于非还原性寡糖和其他中性多糖的微量分析。
