[Comparison of distribution of Ophiopogon japonicas polysaccharide in hearts of normal rats and myocardial ischemic rats].

OBJECTIVE

To investigate the distribution of Ophiopogon Japonicas polysaccharide (OJP) in hearts of normal rats and myocardial ischemic rats, and obtain the further evidences for determining the mechanism of its anti-myocardial ischemia.

METHOD

Hearts of normal and myocardial ischemic groups were obtained at 5, 10, 30 and 60 min after once intravenous administration of OJP with the dose of 50 mg/kg. The concentration of OJP in samples was subsequently determined by HPGPC with a fluorescence detector.

RESULT

The distribution of OJP in hearts of myocardial ischemic rats was much more than that in normal groups at all the sampling times. Moreover, the differences were proved to be significant by SPSS software with the exception of the situation in the 30 min sampling time.

CONCLUSION

The enhanced permeability and retention effect caused by ischemia can overcome the negative effect of the reduction of blood flow on the distribution of OJP from blood into myocardial ischemic zones and actually help OJP target myocardial ischemic zones where it can exert its pharmacological effect directly.