Lin Xiao, Lu Zhiling, Xu Desheng, Feng Yi
Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
Zhongguo Zhong Yao Za Zhi. 2009 Jan;34(2):230-3.
To investigate the distribution of Ophiopogon Japonicas polysaccharide (OJP) in hearts of normal rats and myocardial ischemic rats, and obtain the further evidences for determining the mechanism of its anti-myocardial ischemia.
Hearts of normal and myocardial ischemic groups were obtained at 5, 10, 30 and 60 min after once intravenous administration of OJP with the dose of 50 mg/kg. The concentration of OJP in samples was subsequently determined by HPGPC with a fluorescence detector.
The distribution of OJP in hearts of myocardial ischemic rats was much more than that in normal groups at all the sampling times. Moreover, the differences were proved to be significant by SPSS software with the exception of the situation in the 30 min sampling time.
The enhanced permeability and retention effect caused by ischemia can overcome the negative effect of the reduction of blood flow on the distribution of OJP from blood into myocardial ischemic zones and actually help OJP target myocardial ischemic zones where it can exert its pharmacological effect directly.
研究麦冬多糖（OJP）在正常大鼠和心肌缺血大鼠心脏中的分布情况，为确定其抗心肌缺血作用机制提供进一步证据。
以50mg/kg的剂量一次性静脉注射OJP后，在5、10、30和60分钟时获取正常组和心肌缺血组大鼠的心脏。随后采用带荧光检测器的高效凝胶渗透色谱法（HPGPC）测定样品中OJP的浓度。
在所有采样时间点，心肌缺血大鼠心脏中OJP的分布均远高于正常组。此外，除30分钟采样时间外，经SPSS软件分析，差异具有统计学意义。
缺血引起的通透性增强和滞留效应可克服血流减少对OJP从血液分布到心肌缺血区域的负面影响，实际上有助于OJP靶向心肌缺血区域，在该区域它可直接发挥药理作用。
