Kilstrup Mogens, Hammer Karin, Ruhdal Jensen Peter, Martinussen Jan
Microbial Physiology and Genetics, Technical University of Denmark, Biocentrum-DTU, Building 301, Matematiktorvet, 2800 Kgs Lyngby, Denmark.
FEMS Microbiol Rev. 2005 Aug;29(3):555-90. doi: 10.1016/j.femsre.2005.04.006.
Most metabolic reactions are connected through either their utilization of nucleotides or their utilization of nucleotides or their regulation by these metabolites. In this review the biosynthetic pathways for pyrimidine and purine metabolism in lactic acid bacteria are described including the interconversion pathways, the formation of deoxyribonucleotides and the salvage pathways for use of exogenous precursors. The data for the enzymatic and the genetic regulation of these pathways are reviewed, as well as the gene organizations in different lactic acid bacteria. Mutant phenotypes and methods for manipulation of nucleotide pools are also discussed. Our aim is to provide an overview of the physiology and genetics of nucleotide metabolism and its regulation that will facilitate the interpretation of data arising from genetics, metabolomics, proteomics, and transcriptomics in lactic acid bacteria.
大多数代谢反应通过其对核苷酸的利用、对核苷酸的利用或受这些代谢物的调节而相互关联。在本综述中，描述了乳酸菌中嘧啶和嘌呤代谢的生物合成途径，包括相互转化途径、脱氧核糖核苷酸的形成以及利用外源前体的补救途径。综述了这些途径的酶促调节和遗传调节数据，以及不同乳酸菌中的基因组织。还讨论了突变体表型和操纵核苷酸库的方法。我们的目的是概述核苷酸代谢的生理学和遗传学及其调节，这将有助于解释乳酸菌中遗传学、代谢组学、蛋白质组学和转录组学产生的数据。
