Fermentation Characteristics, Nutrient Content, and Microbial Population of L. Silage Produced with Different Lactic Acid Bacteria Additives.

The aim of this study was to explore the effects of different lactic acid bacteria additives ( or ) on the fermentation quality, chemical composition, in vitro digestibility, bacterial community structure, and predictive function of silage feed. Fresh was wilted overnight, then its moisture content was adjusted between 65 and 70%. The experiment was performed in three groups as follows: (1) the control group (CK group), which lacked a preparation; (2) the () group (LP group), which was inoculated with at 5 × 10 cfu/g FW; and (3) the () group (LB group), which was inoculated with at 5 × 10 cfu/g FW. The results showed that significantly reduced pH and increased lactic acid (LA) content in silage compared with the control. , on the other hand, excelled in reducing ammonia nitrogen (NH-N) content and significantly increased acetic acid (AA) content. At 60 days of fermentation, the CP content was significantly higher ( < 0.05) in the LP and LB groups than in the CK group (19.29 vs. 15.53 and 15.87). At 60 days of fermentation, the CPD was significantly higher ( < 0.05) in the LB group than in the CK and LP groups (57.80 vs. 54.77 and 55.77). The 60-day silage process completely altered the bacterial community of silage. In the fresh samples, the dominant genera were Weissella_A and Pantoea_A. Weissella_A and Pantoea_A were gradually replaced by and after ensiling. After 45 days of fermentation, became the dominant strain in CK, LP and LB groups. Inoculation with altered the succession of the bacterial community from 7 to 15 days of fermentation of . In contrast, inoculation with affected the succession of the bacterial community from 30 to 60 days of fermentation. In silage aged 7 to 60 days, the amino acid metabolic pathway in the LB group remained upregulated. The experimental results revealed that inoculation with had a stronger effect on silage than inoculation with . Thus, should be selected as an additive for silage fermentation in practical production.