He Lichao, Jiang Chao, Dong He, Wang Yinuo, Tang Jiaxin, Hu Mengjie, Luo Junjie, Du Shuai, Jia Yushan, Xiao Yanzi, You Sihan
Key Laboratory of Forage Cultivation, Processing and High Efficient Utilization, Ministry of Agriculture, College of Grassland Science, Inner Mongolia Agricultural University, Hohhot, 010019, China.
College of Agriculture, Grass Industry Collaborative Innovation Research Center, Hulunbuir University, Hulunber, 021000, China.
BMC Microbiol. 2025 May 16;25(1):300. doi: 10.1186/s12866-025-03982-w.
This study aimed to evaluate the effects of cellulase or Lactobacillus plantarum (L. plantarum) on the fermentation characteristics and microbial community structure of the sorghum straw silage. Sorghum straw was treated with the following four experimental conditions: distilled water (control, CK), cellulase (CEL), Lactobacillus plantarum (LP), and a combined treatment of Lactobacillus plantarum with cellulase (LPCEL). These results indicated that the LP treatment could markedly (p < 0.05) preserve the crude protein content compared to that in other treatments, whereas the CEL significantly (p < 0.05) reduced the acid detergent fiber content, while the LPCEL had the highest lactic acid content and lowest pH value. Proteobacteria and Pantoea were identified as the dominant phylum and genus in fresh materials, respectively. This phylum level dominance transitioned to Firmicutes post-treatment, while at the genus level, the community shifted from Pantoea to co-dominance of Lactobacillus and Prevotella, with Lactobacillus being the most abundant in both the CEL and LPCEL treatments. In conclusion, adding L. plantarum and cellulase to sorghum straw can significantly improve the fermentation quality of sorghum straw silage, and improve the nutritional value of silage by affecting the microbial community structure and metabolic pathways.
本研究旨在评估纤维素酶或植物乳杆菌对高粱秸秆青贮发酵特性及微生物群落结构的影响。高粱秸秆采用以下四种试验条件处理：蒸馏水（对照，CK）、纤维素酶（CEL）、植物乳杆菌（LP）以及植物乳杆菌与纤维素酶联合处理（LPCEL）。这些结果表明，与其他处理相比，LP处理能显著（p < 0.05）保留粗蛋白含量，而CEL显著（p < 0.05）降低酸性洗涤纤维含量，LPCEL的乳酸含量最高且pH值最低。变形菌门和泛菌属分别被鉴定为新鲜物料中的优势菌门和优势菌属。处理后，该菌门水平的优势转变为厚壁菌门，而在菌属水平上，群落从泛菌属转变为乳酸杆菌属和普雷沃氏菌属共同占主导，其中乳酸杆菌属在CEL和LPCEL处理中均最为丰富。总之，向高粱秸秆中添加植物乳杆菌和纤维素酶可显著提高高粱秸秆青贮的发酵品质，并通过影响微生物群落结构和代谢途径提高青贮饲料的营养价值。
