Khan Shahid M, Franke-Fayard Blandine, Mair Gunnar R, Lasonder Edwin, Janse Chris J, Mann Matthias, Waters Andrew P
Department of Parasitology, Leiden University Medical Centre, Leiden, The Netherlands.
Cell. 2005 Jun 3;121(5):675-87. doi: 10.1016/j.cell.2005.03.027.
Gametocytes, the precursor cells of malaria-parasite gametes, circulate in the blood and are responsible for transmission from host to mosquito vector. The individual proteomes of male and female gametocytes were analyzed using mass spectrometry, following separation by flow sorting of transgenic parasites expressing green fluorescent protein, in a sex-specific manner. Promoter tagging in transgenic parasites confirmed the designation of stage and sex specificity of the proteins. The male proteome contained 36% (236 of 650) male-specific and the female proteome 19% (101 of 541) female-specific proteins, but they share only 69 proteins, emphasizing the diverged features of the sexes. Of all the malaria life-cycle stages analyzed, the male gametocyte has the most distinct proteome, containing many proteins involved in flagellar-based motility and rapid genome replication. By identification of gender-specific protein kinases and phosphatases and using targeted gene disruption of two kinases, new sex-specific regulatory pathways were defined.
配子体是疟原虫配子的前体细胞，在血液中循环，负责从宿主传播到蚊媒。通过对表达绿色荧光蛋白的转基因寄生虫进行流式分选，以性别特异性方式分离后，利用质谱分析了雄配子体和雌配子体的个体蛋白质组。转基因寄生虫中的启动子标签证实了蛋白质的阶段和性别特异性。雄性蛋白质组包含36%（650个中的236个）雄性特异性蛋白质，雌性蛋白质组包含19%（541个中的101个）雌性特异性蛋白质，但它们仅共享69种蛋白质，强调了两性的不同特征。在分析的所有疟原虫生命周期阶段中，雄配子体具有最独特的蛋白质组，包含许多参与基于鞭毛的运动和快速基因组复制的蛋白质。通过鉴定性别特异性蛋白激酶和磷酸酶，并对两种激酶进行靶向基因破坏，确定了新的性别特异性调节途径。
