Perens Elliot A, Shaham Shai
Laboratory of Developmental Genetics, The Rockefeller University, 1230 York Avenue, New York, New York 10021, USA.
Dev Cell. 2005 Jun;8(6):893-906. doi: 10.1016/j.devcel.2005.03.009.
Sensory organs are often composed of neuronal sensory endings accommodated in a lumen formed by ensheathing epithelia or glia. Here we show that lumen formation in the C. elegans amphid sensory organ requires the gene daf-6. daf-6 encodes a Patched-related protein that localizes to the luminal surfaces of the amphid channel and other C. elegans tubes. While daf-6 mutants display only amphid lumen defects, animals defective for both daf-6 and the Dispatched gene che-14 exhibit defects in all tubular structures that express daf-6. Furthermore, DAF-6 protein is mislocalized, and lumen morphogenesis is abnormal, in mutants with defective sensory neuron endings. We propose that amphid lumen morphogenesis is coordinated by neuron-derived cues and a DAF-6/CHE-14 system that regulates vesicle dynamics during tubulogenesis.
感觉器官通常由容纳在由包被上皮或神经胶质形成的管腔中的神经元感觉末梢组成。在这里，我们表明秀丽隐杆线虫嗅觉感觉器官中的管腔形成需要daf-6基因。daf-6编码一种与Patched相关的蛋白质，该蛋白质定位于嗅觉通道和其他秀丽隐杆线虫管道的管腔表面。虽然daf-6突变体仅表现出嗅觉管腔缺陷，但daf-6和Dispatched基因che-14均有缺陷的动物在所有表达daf-6的管状结构中都表现出缺陷。此外，在感觉神经元末梢有缺陷的突变体中，DAF-6蛋白定位错误，管腔形态发生异常。我们提出，嗅觉管腔形态发生是由神经元衍生的信号和一个在管状发生过程中调节囊泡动力学的DAF-6/CHE-14系统协调的。
