Grandjean-Laquerriere Alexia, Laquerriere Patrice, Jallot Edouard, Nedelec Jean-Marie, Guenounou Moncef, Laurent-Maquin Dominique, Phillips Terry M
Ultramicro Analytical Immunochemistry Resource, Division of Bioengineering & Physical Science, Office of Research Services, National Institutes of Health, Bethesda, MD 20892, USA.
Biomaterials. 2006 Jun;27(17):3195-200. doi: 10.1016/j.biomaterials.2006.01.024. Epub 2006 Feb 17.
A possible complication associated with the implantation of hydroxyapatite (HA)-based prosthesis is the release of particles. These particles can be phagocyted by monocytes that are among the first cells to colonize the inflammatory site. The activated monocytes produce inflammatory mediators such as cytokines that cause osteoclasts activation. The present work, describes studies on the effect of sol-gel derived zinc-substituted HA particles with various zinc substitution percentages (0.5-2%) on cytokine production (TNF-alpha, IL-1beta, IL-6, IL-10, and IL-8) by both LPS-stimulated or unstimulated human monocytes. Our data demonstrates that the zinc has an effect on cytokines production. It decreases the production of TNF-alpha and increases the production of IL-8 by unstimulated cells. Using LPS-stimulated cells, it decreases the production of inflammatory cytokines and increases the production of anti-inflammatory cytokine (IL-10), indicating that zinc-substituted hydroxyapatite has favourable effects on the cytokines production by monocytes.
与植入基于羟基磷灰石（HA）的假体相关的一种可能并发症是颗粒释放。这些颗粒可被单核细胞吞噬，单核细胞是最早在炎症部位定植的细胞之一。活化的单核细胞产生炎症介质，如导致破骨细胞活化的细胞因子。本研究描述了对溶胶 - 凝胶法制备的不同锌取代百分比（0.5 - 2%）的锌取代HA颗粒对脂多糖刺激或未刺激的人单核细胞产生细胞因子（TNF-α、IL-1β、IL-6、IL-10和IL-8）的影响的研究。我们的数据表明锌对细胞因子的产生有影响。它可降低未刺激细胞中TNF-α的产生，并增加IL-8的产生。使用脂多糖刺激的细胞时，它可降低炎性细胞因子的产生并增加抗炎细胞因子（IL-10）的产生，表明锌取代的羟基磷灰石对单核细胞产生细胞因子具有有利影响。
