高效液相色谱/电喷雾离子阱质谱法测定人血浆中青藤碱的含量
Determination of sinomenine in human plasma by HPLC/ESI/ion trap mass spectrum.
作者信息
Yao Yong-Mei, Tan Zhi-Rong, Hu Zhe-Yi, Guo Xin, Cheng Ze-Neng, Wang Lian-Sheng, Zhou Hong-Hao
机构信息
Pharmacogenetics Research Institute, Institute of Clinical Pharmacology, Xiang-Ya School of Medicine, Central South University, Changsha, Hunan 410078, P.R. China.
出版信息
Clin Chim Acta. 2005 Jun;356(1-2):212-7. doi: 10.1016/j.cccn.2005.01.018. Epub 2005 Apr 1.
BACKGROUND
Sinomenine is a pure alkaloid with a variety of pharmacological actions including anti-inflammation, immuno-suppression, analgesia, antihypertension, and antiarrhythmia. Methods have been developed to measure sinomenine in rats and rabbits through high-performance liquid chromatography (HPLC). But only one report described a method for determination of sinomenine in humans.
METHOD
We developed a method to quantitate sinomenine in human plasma based on liquid chromatography-ion trap mass spectrometry coupled with electrospray ionization (HPLC/ESI/ion trap mass spectrum).
RESULTS
The calibration curve (r2=0.9993) of sinomenine was established with standard solutions. The mean inter-day and intra-day CVs<15%. The limit of detection for sinomenine was 0.5 ng/ml. The relative recovery of high, middle and low concentration was 99%, 103%, and 118%, respectively.
CONCLUSION
The method is simple, rapid, sensitive, specific, and accurate enough for determination of sinomenine in human plasma and may be used to investigate the metabolism and pharmacokinetics of sinomenine.
背景
青藤碱是一种纯生物碱，具有多种药理作用，包括抗炎、免疫抑制、镇痛、抗高血压和抗心律失常。已经开发出通过高效液相色谱法（HPLC）测定大鼠和兔子体内青藤碱的方法。但仅有一篇报道描述了一种测定人体中青藤碱的方法。
方法
我们开发了一种基于液相色谱-离子阱质谱联用并结合电喷雾电离（HPLC/ESI/离子阱质谱）定量人体血浆中青藤碱的方法。
结果
用标准溶液建立了青藤碱的校准曲线（r2 = 0.9993）。日间和日内平均变异系数<15%。青藤碱的检测限为0.5 ng/ml。高、中、低浓度的相对回收率分别为99%、103%和118%。
结论
该方法简单、快速、灵敏、特异且准确，足以用于测定人体血浆中的青藤碱，可用于研究青藤碱的代谢和药代动力学。
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