Ditt Renata Fava, Nester Eugene, Comai Luca
Department of Biology, University of Washington, Seattle, Box 355325, WA 98195, USA.
FEMS Microbiol Lett. 2005 Jun 15;247(2):207-13. doi: 10.1016/j.femsle.2005.05.010.
We previously identified changes in gene expression in Ageratum conyzoides plant cells inoculated with Agrobacterium tumefaciens by using cDNA-AFLP. Here, we show that a subset of defense-related genes is differentially regulated by an Agrobacterium attachment-deficient mutant. The expression pattern triggered by this mutant is similar to that induced by inoculation with non-pathogenic bacteria. We also observed that the expression level of the defense genes was inversely correlated with the efficiency of transformation by Agrobacterium. We propose that the plant defense system has an important role in controlling infection and transformation and that Agrobacterium may dampen some plant defense responses.
我们之前通过cDNA-AFLP技术鉴定了接种根癌农杆菌的藿香蓟植物细胞中基因表达的变化。在此，我们表明一组与防御相关的基因受到根癌农杆菌附着缺陷型突变体的差异调控。该突变体引发的表达模式与接种非致病细菌诱导的模式相似。我们还观察到防御基因的表达水平与根癌农杆菌的转化效率呈负相关。我们提出植物防御系统在控制感染和转化中具有重要作用，并且根癌农杆菌可能会抑制一些植物防御反应。
