Kim Su-Sung, Shin Dong-Il, Park Hee-Sung
Department of Biotechnology, Catholic University of Daegu, Kyungsan, Kyungbuk, Korea.
Biotechnol Lett. 2007 Jun;29(6):965-9. doi: 10.1007/s10529-007-9326-5. Epub 2007 Feb 20.
A pollen-based transient expression system has been developed. Lily pollen grains, wounded by vigorous shaking in the presence of aluminum oxide particles, were transformed by infiltration with Agrobacterium tumefaciens LBA4404 cells harboring the beta-glucuronidase (GUS) gene construct, pBI121. In histochemical and fluorometric GUS analysis, the wounding processes allowed efficient transformation and, in cDNA blot hybridization, GUS mRNA synthesis was clearly detected. Lily pollen with appropriate wounds, therefore, can be used conveniently for the rapid production of recombinant proteins.
一种基于花粉的瞬时表达系统已被开发出来。在氧化铝颗粒存在的情况下，通过剧烈振荡使百合花粉粒受伤，然后用携带β-葡萄糖醛酸酶（GUS）基因构建体pBI121的根癌农杆菌LBA4404细胞进行浸润转化。在组织化学和荧光GUS分析中，创伤过程允许高效转化，并且在cDNA印迹杂交中，清楚地检测到了GUS mRNA的合成。因此，具有适当创伤的百合花粉可方便地用于快速生产重组蛋白。
