Department of Biological Sciences, University of Lethbridge , Lethbridge, Canada.
Plant Signal Behav. 2020 Aug 2;15(8):1780037. doi: 10.1080/15592324.2020.1780037. Epub 2020 Jun 17.
In plant biology, transient expression analysis plays a vital role to provide a fast method to study the gene of interest. In this study, we report a rapid and efficient method for transient expression in seedlings using -mediated transformation. strain EHA105 carrying the pCAMBIA1301 construct with A gene was used to transform cannabis seedlings and the GUS assay (a measurement of β-glucuronidase activity) was used to detect the A expression. In the current study, we have also established a rapid germination protocol for cannabis seeds. The all three steps seed sterilization, germination and seedlings development were carried out in a 1% HO solution. Transient transformation revealed that both cotyledons and young true leaves are amenable to transformation. Compared with tobacco (), cannabis seedlings were less susceptible to transformation with . Susceptibility to transformation also varied with the different cannabis varieties. The method established in this study has the potential to be an important tool for gene function studies and genetic improvement in cannabis.
在植物生物学中，瞬时表达分析对于提供一种快速研究目的基因的方法至关重要。在本研究中，我们报告了一种使用农杆菌介导转化在大麻幼苗中进行瞬时表达的快速高效方法。携带 A 基因的 pCAMBIA1301 构建体的 EHA105 菌株被用于转化大麻幼苗，并使用 GUS 测定（β-葡萄糖醛酸酶活性的测量）来检测 A 的表达。在本研究中，我们还建立了一种大麻种子的快速萌发方案。所有三个种子消毒、萌发和幼苗发育步骤都在 1%HO 溶液中进行。瞬时转化表明，子叶和幼嫩真叶都适合转化。与烟草（）相比，大麻幼苗对 的转化敏感性较低。对 转化的敏感性也随不同的大麻品种而变化。本研究中建立的方法有可能成为大麻基因功能研究和遗传改良的重要工具。
