蛋白质中所有主链极性基团都会形成氢键吗？
Do all backbone polar groups in proteins form hydrogen bonds?
作者信息
Fleming Patrick J, Rose George D
机构信息
T. C. Jenkins Department of Biophysics, Johns Hopkins University, Baltimore, MD 21218, USA.
出版信息
Protein Sci. 2005 Jul;14(7):1911-7. doi: 10.1110/ps.051454805. Epub 2005 Jun 3.
Abstract
Evidence from proteins and peptides supports the conclusion that intrapeptide hydrogen bonds stabilize the folded form of proteins. Paradoxically, evidence from small molecules supports the opposite conclusion, that intrapeptide hydrogen bonds are less favorable than peptide-water hydrogen bonds. A related issue-often lost in this debate about comparing peptide-peptide to peptide- water hydrogen bonds-involves the energetic cost of an unsatisfied hydrogen bond. Here, experiment and theory agree that breaking a hydrogen bond costs between 5 and 6 kcal/mol. Accordingly, the likelihood of finding an unsatisfied hydrogen bond in a protein is insignificant. This realization establishes a powerful rule for evaluating protein conformations.
摘要
来自蛋白质和肽的证据支持这样的结论
肽内氢键稳定了蛋白质的折叠形式。矛盾的是，来自小分子的证据却支持相反的结论，即肽内氢键不如肽与水之间的氢键有利。一个相关问题——在这场关于比较肽-肽氢键与肽-水氢键的争论中常常被忽视——涉及未满足氢键的能量成本。在此，实验和理论一致认为，打破一个氢键的成本在5至6千卡/摩尔之间。因此，在蛋白质中发现未满足氢键的可能性微乎其微。这一认识确立了一条评估蛋白质构象的有力规则。
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