Cornelissen B J, Horowitz J, van Kan J A, Goldberg R B, Bol J F
Department of Biochemistry, Leiden University, The Netherlands.
Nucleic Acids Res. 1987 Sep 11;15(17):6799-811. doi: 10.1093/nar/15.17.6799.
Infection of Samsun NN tobacco with tobacco mosaic virus (TMV) was found to induce the synthesis of mRNA encoding a basic protein with a 67% amino acid sequence homology to the known acidic pathogenesis-related (PR) proteins 1a, 1b and 1c. By Southern blot hybridization it was shown that the tobacco genome contains at least eight genes for acidic PR-1 proteins and a similar number of genes encoding the basic homologues. Clones corresponding to three of the genes for acidic PR-1 proteins were isolated from a genomic library of Samsun NN tobacco. The nucleotide sequence of these genes and their flanking sequences were determined. One clone was found to correspond to the PR-1a gene; the two other clones do not correspond to known TMV-induced PR-1 mRNA's and may represent silent genes. Compared to the PR-1a gene, these genes contain an insertion or deletion in the putative promoter region and mutations affecting the PR-1 reading frame.
研究发现，烟草花叶病毒（TMV）感染萨姆松NN烟草会诱导合成一种编码碱性蛋白的mRNA，该碱性蛋白与已知的酸性病程相关（PR）蛋白1a、1b和1c具有67%的氨基酸序列同源性。通过Southern印迹杂交表明，烟草基因组至少包含8个酸性PR-1蛋白基因以及数量相似的编码碱性同源物的基因。从萨姆松NN烟草的基因组文库中分离出了与三个酸性PR-1蛋白基因相对应的克隆。测定了这些基因及其侧翼序列的核苷酸序列。发现一个克隆对应于PR-1a基因；另外两个克隆与已知的TMV诱导的PR-1 mRNA不对应，可能代表沉默基因。与PR-1a基因相比，这些基因在假定的启动子区域存在插入或缺失，并且存在影响PR-1阅读框的突变。
