Zhang Huai-Xia, Feng Xiao-Hui, Ali Muhammad, Jin Jing-Hao, Wei Ai-Min, Khattak Abdul Mateen, Gong Zhen-Hui
College of Horticulture, Northwest A&F University, Yangling, China.
Tianjin Vegetable Research Center, Tianjin Academy of Agricultural Sciences, Tianjin, China.
Front Plant Sci. 2020 Feb 26;11:183. doi: 10.3389/fpls.2020.00183. eCollection 2020.
Little information is available on the role of Squamosa promoter binding protein (SBP)-box genes in pepper plants. This family of genes is known to have transcription characteristics specific to plants and to regulate plant growth, development, stress responses, and signal transduction. To investigate their specific effects in pepper (), we screened pepper SBP-box family genes ( genes) for () resistance genes using virus-induced gene silencing. , , , and , which are associated with plant defense responses against , were obtained from among fifteen identified genes. The function of was identified in pepper defense response against infection in particular. CaSBP08 protein was localized to the nucleus. Silencing of enhanced resistance to infection. Following inoculation, the malondialdehyde content, peroxidase activity, and disease index percentage of the -silenced plants decreased compared to the control. Additionally, the expression levels of other defense-related genes, especially those of and , were more strongly induced in -silenced plants than in the control. However, overexpression in enhanced susceptibility to infection. This work provides a foundation for the further research on the role of genes in plant defense responses against infection.
关于鳞状启动子结合蛋白（SBP）-盒基因在辣椒植株中的作用，目前所知信息较少。已知该基因家族具有植物特有的转录特性，并能调控植物的生长、发育、应激反应和信号转导。为了研究它们在辣椒中的具体作用，我们利用病毒诱导基因沉默技术筛选了辣椒SBP-盒家族基因（基因）以寻找抗疫霉（）的抗性基因。从15个已鉴定的基因中获得了与植物对疫霉的防御反应相关的、、、和。特别鉴定了在辣椒对疫霉感染的防御反应中的功能。CaSBP08蛋白定位于细胞核。的沉默增强了对疫霉感染的抗性。接种疫霉后，与对照相比，沉默植株的丙二醛含量、过氧化物酶活性和病情指数百分比均降低。此外，其他防御相关基因的表达水平，尤其是和的表达水平，在沉默植株中比在对照中更强烈地被诱导。然而，在中的过表达增强了对疫霉感染的易感性。这项工作为进一步研究基因在植物对疫霉感染的防御反应中的作用奠定了基础。
