van Tol R G, van Vloten-Doting L
Eur J Biochem. 1979 Feb 1;93(3):461-8. doi: 10.1111/j.1432-1033.1979.tb12844.x.
Translation of alfalfa mosaic virus (AMV) RNAs in the mRNA-dependent rabbit reticulocyte cell-free system was examined using different RNA concentrations. The pattern of products synthesized under the direction of AMV RNA 2, 3 and 4 was not or almost not influenced by their concentration. However, depending on the RNA 1 concentration either a very large protein of Mr 115,000 or a mixture of two smaller proteins, Mr 58,000 and 62,000 respectively, was formed. These three proteins represent overlapping peptide chains with identical N-termini. Addition of the cap analogue 7-methylguanosine 5'-monophosphate (m7GMP) or AMV RNA 3 stimulated the production of the 115,000-Mr protein at the expense of the 58,000-Mr and 62,000-Mr proteins. Both m7GMP and RNA 3 probably reduce the active concentration of RNA 1 by competing for (a) cellular component(s) necessary for translation. These experimental results suggest that the rate of translation beyond the C termini of the 58,000-Mr and 62,000-Mr proteins is reduced or completely inhibited owing to the limited availability of the succeeding tRNA(s).
在依赖mRNA的兔网织红细胞无细胞体系中，使用不同的RNA浓度检测了苜蓿花叶病毒（AMV）RNA的翻译情况。在AMV RNA 2、3和4指导下合成的产物模式不受其浓度影响或几乎不受影响。然而，根据RNA 1的浓度，要么形成一种分子量为115,000的非常大的蛋白质，要么形成两种较小蛋白质的混合物，其分子量分别为58,000和62,000。这三种蛋白质代表具有相同N端的重叠肽链。添加帽类似物7-甲基鸟苷5'-单磷酸（m7GMP）或AMV RNA 3以牺牲分子量为58,000和62,000的蛋白质为代价刺激了分子量为115,000的蛋白质的产生。m7GMP和RNA 3可能都通过竞争翻译所需的一种或多种细胞成分来降低RNA 1的活性浓度。这些实验结果表明，由于后续tRNA可用性有限，分子量为58,000和62,000的蛋白质C端之后的翻译速率降低或完全受到抑制。
