Teles Helder L, Hemerly Jefferson P, Paulettit Patrícia M, Pandolfi José R C, Araujot Angela R, Valentini Sandro R, Young M Claudia M, Bolzani Vanderlan S, Silva Dulce H S
Instituto de Química - UNESP, Araraquara, C.P. 355, 14800-900.
Nat Prod Res. 2005 Jun;19(4):319-23. doi: 10.1080/14786410412331271979.
An ethanolic extract from the stems of Styrax camporum Pohl (Styracaceae), a plant traditionally used for gastrointestinal diseases, was fractionated and subjected to flash chromatography and afforded two benzofuran lignans, egonol and homoegonol, and one furofuran lignan, (+/-)syringaresinol, which were identified by spectral data interpretation. Their cytotoxic activities against Hep-2 (larynx epidermoid carcinoma), HeLa (human cervix carcinoma) and C6 (rat glioma) cell lines were evaluated using the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide) assay at several concentrations for 24h. Activities could be observed for egonol against C6 (IC50 = 3.2 microg/mL) and Hep-2 (IC50 = 3.6 microg/mL) cell lines, and for homoegonol against C6 (IC50 = 4.9 microg/mL) and HeLa (IC50 = 5.3 microg/mL) cells.
安息香科植物Styrax camporum Pohl的茎的乙醇提取物，这种植物传统上用于治疗胃肠道疾病，经过分馏并进行快速柱色谱法，得到了两种苯并呋喃木脂素，即紫丁香醇和高紫丁香醇，以及一种呋喃呋喃木脂素，（±）丁香树脂醇，通过光谱数据解析对它们进行了鉴定。使用MTT（3 -（4,5 - 二甲基噻唑 - 2 - 基）-2,5 - 二苯基 - 2H - 四氮唑溴盐）法在几种浓度下对它们针对Hep - 2（喉表皮样癌）、HeLa（人宫颈癌）和C6（大鼠胶质瘤）细胞系的细胞毒性活性进行了24小时评估。观察到紫丁香醇对C6（IC50 = 3.2微克/毫升）和Hep - 2（IC50 = 3.6微克/毫升）细胞系有活性，高紫丁香醇对C6（IC50 = 4.9微克/毫升）和HeLa（IC50 = 5.3微克/毫升）细胞有活性。
