de Oliveira Pollyanna Francielli, Damasceno Jaqueline Lopes, Bertanha Camila Spereta, Araújo Alba Regina Barbosa, Pauletti Patrícia Mendonça, Tavares Denise Crispim
Universidade de Franca, Avenida Dr. Armando Salles de Oliveira, 201 - Parque Universitário, Franca, São Paulo, 14404-600, Brazil.
Cytotechnology. 2016 Aug;68(4):1597-602. doi: 10.1007/s10616-015-9864-y. Epub 2015 Mar 21.
The benzofuran lignans egonol and homoegonol are found in all species of the genus Styrax. Since natural products are important sources of new anticancer drugs, this study evaluated the cytotoxic activity of a hydroalcoholic extract of the stems of S. camporum (SCHE) and their chemical markers, egonol (EG) and homoegonol (HE), against different tumor cell lines (B16F10, MCF-7, HeLa, HepG2, and MO59J). A normal human cell line (GM07492A) was included. Cytotoxic activity was evaluated at different treatment times (24, 48 and 72 h) using the XTT assay. More effective results were observed after 72 h of treatment. The lowest IC50 values were found for the HepG2 cell line, ranging from 11.2 to 55.0 µg/mL. The combination of EG and HE exerted higher cytotoxic activity than SCHE or treatment with either lignan alone, with the lowest IC50 (13.31 µg/mL) being observed for the MCF-7 line. Furthermore, treatment with these lignans was significantly more cytotoxic for some tumor cell lines compared to the normal cell line, GM07492A, indicating selectivity. These results suggest that these lignans may be used to treat cancer without affecting normal cells.
苯并呋喃木脂素（如紫铆因和高紫铆因）存在于安息香属的所有物种中。由于天然产物是新型抗癌药物的重要来源，本研究评估了安息香（Styrax camporum）茎部水醇提取物（SCHE）及其化学标志物紫铆因（EG）和高紫铆因（HE）对不同肿瘤细胞系（B16F10、MCF-7、HeLa、HepG2和MO59J）的细胞毒性活性。还纳入了一种正常人细胞系（GM07492A）。使用XTT法在不同处理时间（24、48和72小时）评估细胞毒性活性。处理72小时后观察到更有效的结果。HepG2细胞系的IC50值最低，范围为11.2至55.0μg/mL。EG和HE的组合比SCHE或单独使用任何一种木脂素处理具有更高的细胞毒性活性，MCF-7细胞系的IC50最低（13.31μg/mL）。此外，与正常细胞系GM07492A相比，这些木脂素对某些肿瘤细胞系的细胞毒性明显更高，表明具有选择性。这些结果表明，这些木脂素可用于治疗癌症而不影响正常细胞。
