Chen Jing, Wang Jia-han, Zhuang Hong-xing, Ren Jia-liang, Li Zhi-qing, Yi Chao-hui
Department of Burns, Nanfang Hospital, South Medical University, Guangzhou 510515, P.R. China.
Zhonghua Shao Shang Za Zhi. 2005 Apr;21(2):119-21.
To explore the relationship between neuropeptide substance P (SP) and wound healing in scalded rats.
(1) Scalded rats with different degrees of scald injury were employed as the experimental model and were sacrificed at 24 post scald hour (PSH), and on 3, 7 and 14 post scald days (PSD). The SP content in the wound was detected with radioimmunoassay method. (2) The murine granulation tissue fibroblasts (GTF) were cultured with different culture media, and divided into control, SP and Spantide (SP receptor antagonism) groups. The effects of SP and Spantide on the cellular activity and apoptotic rate of murine GTF were assessed in vitro.
There was significant difference of the SP content among the superficial (145 +/- 78) ng/g, partial (94 +/- 48 ng/g) and full thickness (53 +/- 27 ng/g) scald wounds at 24 PSH (P < 0.01), while the SP content in partial thickness burn wound on 3 and 7 PSD obviously increased; and that in deep partial thickness burn wound obviously increased on 7 and 14 PSD. But the SP content remained unchanged in full thickness scald wound. (2) SP could promote the activity of GTF and inhibit its apoptosis (The GTF activity in control, SP groups were 0.21 +/- 0.05, 0.36 +/- 0.07, respectively, P < 0.01). Spantide could inhibit the interaction between SP and GTF.
SP can promote GTF proliferation, and the SP content in wound is closely associated with the depth of the injury and wound healing capacity.
探讨神经肽P物质（SP）与烫伤大鼠伤口愈合之间的关系。
（1）采用不同程度烫伤的大鼠作为实验模型，于烫伤后24小时（PSH）、烫伤后3天、7天和14天（PSD）处死。用放射免疫分析法检测伤口中的SP含量。（2）用不同培养基培养小鼠肉芽组织成纤维细胞（GTF），分为对照组、SP组和Spantide（SP受体拮抗剂）组。在体外评估SP和Spantide对小鼠GTF细胞活性和凋亡率的影响。
（1）在烫伤后24小时，浅度（145±78）ng/g、深度（94±48 ng/g）和全层（53±27 ng/g）烫伤伤口的SP含量有显著差异（P<0.01），而深度烫伤伤口在烫伤后3天和7天的SP含量明显增加；深Ⅱ度烫伤伤口在烫伤后7天和14天明显增加。但全层烫伤伤口的SP含量保持不变。（2）SP可促进GTF的活性并抑制其凋亡（对照组、SP组的GTF活性分别为0.21±0.05、0.36±0.07，P<0.01）。Spantide可抑制SP与GTF之间的相互作用。
SP可促进GTF增殖，伤口中的SP含量与损伤深度和伤口愈合能力密切相关。
