[海水浸泡对烫伤大鼠创面炎症及愈合的影响]

[Influence of sea water immersion on inflammation and healing of the wounds in scalded rats].

作者信息

Fan Zi-feng, Wang Jia-han, Li Zhi-qing, Yi Chao-hui

机构信息

Department of Burns, Nanfang Hospital, Southern Medical University, Guangzhou 510515, PR China.

出版信息

Zhonghua Shao Shang Za Zhi. 2006 Jun;22(3):215-7.

PMID:16964652

Abstract

OBJECTIVE

To investigate the influence of sea water immersion on inflammation and healing of the wounds in scalded rats.

METHODS

One hundred and forty-four male Wistar rats with 10% TBSA superficial partial-thickness scald were randomly divided into A (n=72, with scald) and B (n=72, with seawater immersion for 4hrs immediately after scald) groups. The serum contents of K+, Na+, Cl- were determined at 0 post-scald hour (PSH), 6PSH, 12PSH and 24 PSH with electrocyte analysis apparatus, and the changes in serum interleukin-6 (IL-6) and tumor necrosis factor (TNF-alpha) levels were determined at 0 PSH, 6PSH and 12 PSH with enzyme-linked immunosorbent assay (ELISA). The histopathological changes in the rats of the two groups were observed, and wound healing time was respectively calculated.

RESULTS

The serum contents of K+, Na+, Cl- in B group were obviously higher than those in A group. And the serum content of TNF-alpha and IL-6 in B group at 6 PSH [(140 +/- 22) ng/L, (160 +/- 41) ng/L] were significantly higher than those before scald [(29 +/- 15) ng/L, (62 +/- 17)] ng/L and in A group [(120 +/- 12) ng/L, (124 +/- 22) ng/L, ( P < 0.05)]. Compared with A group, re-epithelization of the wound differentiation in all layers of epidermis were delayed in B group, with more severe wound swelling, exudation, and topical inflammatory response. The wound healing time in B group was (16.3 +/- 1.6) d, which was obviously longer than that in A group [(14.1 +/- 1.8) d, P < 0.05)].

CONCLUSION

Sea water immersion combined with scald injury can aggravate the inflammatory response of the wound and delay the wound healing process.

摘要

目的

探讨海水浸泡对烫伤大鼠创面炎症及愈合的影响。

方法

将144只10%体表面积浅Ⅱ度烫伤的雄性Wistar大鼠随机分为A组（n = 72，烫伤组）和B组（n = 72，烫伤后立即海水浸泡4小时组）。采用电解质分析仪于烫伤后0小时（PSH）、6PSH、12PSH和24PSH测定血清K⁺、Na⁺、Cl⁻含量，采用酶联免疫吸附测定（ELISA）法于0PSH、6PSH和12PSH测定血清白细胞介素-6（IL-6）和肿瘤坏死因子（TNF-α）水平变化。观察两组大鼠的组织病理学变化，并分别计算创面愈合时间。

结果

B组血清K⁺、Na⁺、Cl⁻含量明显高于A组。B组6PSH时血清TNF-α和IL-6含量[（140±22）ng/L，（160±41）ng/L]显著高于烫伤前[（29±15）ng/L，（62±17）ng/L]及A组[（120±12）ng/L，（124±22）ng/L，P<0.05]。与A组相比，B组创面各层表皮分化再上皮化延迟，创面肿胀、渗出及局部炎症反应更严重。B组创面愈合时间为（16.3±1.6）天，明显长于A组[（14.1±1.8）天，P<0.05]。