Levine Martin, Owen Willis L, Avery Kevin T
Departments of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, P.O. Box 26901, Oklahoma City, Oklahoma 73190, USA.
Clin Diagn Lab Immunol. 2005 Jun;12(6):764-9. doi: 10.1128/CDLI.12.6.764-769.2005.
Fluoridated dentifrices reduce dental caries in subjects who perform effective oral hygiene. Actinomyces naeslundii increases in teeth-adherent microbial biofilms (plaques) in these subjects, and a well-characterized serum immunoglobulin G (IgG) antibody response (Actinomyces antibody [A-Ab]) is also increased. Other studies suggest that a serum IgG antibody response to streptococcal d-alanyl poly(glycerophosphate) (S-Ab) may indicate caries experience associated strongly with gingival health and exposure to fluoridated water. The aim of this study was to investigate relationships between A-Ab response, oral hygiene, S-Ab response, and caries experience. Measurements were made of A-Ab and S-Ab concentrations, caries experience (number of decayed, missing, and filled teeth [DMFT], number of teeth surfaces [DMFS], and number of decayed teeth needing treated [DT]), exposure to fluoridated water (Flu), mean clinical pocket depth (PD; in millimeters), and extent of plaque (PL) and gingival bleeding on probing (BOP). A-Ab concentration, the dependent variable in a multiple regression analysis, increased with S-Ab concentration and decreased with PL and DMFT adjusted for Flu (R(2) = 0.51, P < 0.002). Residual associations with age, DMFS, DT, and BOP were not significant. In addition, an elevated A-Ab response, defined from immunoprecipitation and immunoassay measurements, indicated a significant, 30% reduction in DMFT after adjustment for significant age and Flu covariance (analysis of variance with covariance F statistic = 10.6, P < 0.003; S-Ab response and interactions not significant). Thus, an elevated A-Ab response indicates less caries in subjects performing effective oral hygiene using fluoridated dentifrices. Conversely, a low A-Ab response is suggestive of decreased A. naeslundii binding to saliva-coated apatite and greater caries experience, as reported by others.
含氟牙膏可减少有效进行口腔卫生护理的人群的龋齿。在这些人群中，内氏放线菌在牙齿附着的微生物生物膜（菌斑）中增多，同时血清免疫球蛋白G（IgG）抗体反应（放线菌抗体[A-Ab]）也增强。其他研究表明，针对链球菌D-丙氨酰聚甘油磷酸酯的血清IgG抗体反应（S-Ab）可能表明与牙龈健康及接触含氟水密切相关的龋齿经历。本研究的目的是调查A-Ab反应、口腔卫生、S-Ab反应与龋齿经历之间的关系。测量了A-Ab和S-Ab浓度、龋齿经历（龋失补牙数[DMFT]、牙面数[DMFS]以及需要治疗的龋齿数[DT]）、接触含氟水情况（Flu）、平均临床龈沟深度（PD；以毫米为单位）、菌斑范围（PL）以及探诊时牙龈出血情况（BOP）。在多元回归分析中作为因变量的A-Ab浓度，随S-Ab浓度升高而升高，经Flu校正后随PL和DMFT降低（R² = 0.51，P < 0.002）。与年龄、DMFS、DT和BOP的残余关联不显著。此外，根据免疫沉淀和免疫测定测量结果定义的升高的A-Ab反应表明，在对显著的年龄和Flu协变量进行校正后，DMFT显著降低30%（协方差分析的F统计量 = 10.6，P < 0.003；S-Ab反应及相互作用不显著）。因此，升高的A-Ab反应表明使用含氟牙膏有效进行口腔卫生护理的人群龋齿较少。相反，正如其他人所报道的，低A-Ab反应提示内氏放线菌与唾液包被的磷灰石的结合减少以及龋齿经历增加。
