Cui Yali, Wang Yani, Hui Wenli, Zhang Zhifeng, Xin Xiaofang, Chen Chao
Biochip Research and Development Center, Northwest University, Xi'an 710069, PR China.
Biomed Microdevices. 2005 Jun;7(2):153-6. doi: 10.1007/s10544-005-1596-x.
Fe3O4/Au (GoldMag) particles with core/shell structure were synthesized by reduction of Au3+ with hydroxylamine in the presence of Fe3O4. The synthesized particles have an average size smaller than 100 nm in diameter with of superparemagnetic properties due to their Fe oxide cores. The particles show optical features with a plasmon resonance peak from 550, 570 to 590 nm correlating with increasing diameters from 50 nm, 70 nm to 100 nm. The GoldMag particles need only a single step for antibody immobilization and have high binding capacity for antibodies. These advantages permit improved methods of isolating and detecting biomolecules.
通过在四氧化三铁存在下用羟胺还原金离子，合成了具有核壳结构的四氧化三铁/金（金磁）颗粒。合成的颗粒平均直径小于100纳米，由于其氧化铁核而具有超顺磁性。这些颗粒在550、570至590纳米处呈现等离子体共振峰的光学特征，这与直径从50纳米、70纳米增加到100纳米相关。金磁颗粒固定抗体仅需一步，且对抗体具有高结合能力。这些优点使得分离和检测生物分子的方法得到改进。
