McCall Andrew D, Baker Olga J
Department of Oral Biology, School of Dental Medicine, The State University of New York at Buffalo, Buffalo, New York (ADM)
School of Dentistry, University of Utah, Salt Lake City, Utah (OJB)
J Histochem Cytochem. 2015 May;63(5):340-9. doi: 10.1369/0022155415573323. Epub 2015 Jan 30.
Angiogenesis has been proposed to play a role in the inflammation observed in Sjögren's Syndrome (SS). However, no studies have validated the degree of angiogenesis in salivary glands with SS. Therefore, the goal of this study was to determine the presence and localization of angiogenesis and lymphangiogenesis in salivary glands with SS. We used frozen tissue sections from human minor salivary glands (hMSG) with and without SS in our analyses. To investigate signs of angiogenesis, hMSG tissue lysates were used to detect levels of the pro-angiogenic protein vascular endothelial growth factor (VEGF) by western blot analyses. Additionally, we labeled blood vessels using antibodies specific to platelet endothelial cell adhesion molecule-1 (PECAM-1) and von Willebrand Factor (vWF) to determine blood vessel organization and volume fraction using fluorescence microscopy. Lymphatic vessel organization and volume fraction were determined using antibodies specific to lymphatic vessel endothelial hyaluronan receptor (LYVE-1). Our results suggest that expression levels of VEGF are decreased in hMSG with SS as compared with controls. Interestingly, there were no significant differences in blood or lymphatic vessel organization or volume fraction between hMSG with and without SS, suggesting that angiogenesis and lymphangiogenesis have little impact on the progression of SS.
血管生成被认为在干燥综合征（SS）中观察到的炎症中起作用。然而，尚无研究验证SS患者唾液腺中血管生成的程度。因此，本研究的目的是确定SS患者唾液腺中血管生成和淋巴管生成的存在及定位。我们在分析中使用了来自有或无SS的人类小唾液腺（hMSG）的冷冻组织切片。为了研究血管生成的迹象，通过蛋白质印迹分析，使用hMSG组织裂解物检测促血管生成蛋白血管内皮生长因子（VEGF）的水平。此外，我们使用血小板内皮细胞黏附分子-1（PECAM-1）和血管性血友病因子（vWF）特异性抗体标记血管，以使用荧光显微镜确定血管组织和体积分数。使用淋巴管内皮透明质酸受体（LYVE-1）特异性抗体确定淋巴管组织和体积分数。我们的结果表明，与对照组相比，SS患者hMSG中VEGF的表达水平降低。有趣的是，有或无SS的hMSG之间在血管或淋巴管组织或体积分数方面没有显著差异，这表明血管生成和淋巴管生成对SS的进展影响很小。
