Iraz Mustafa, Erdogan Hasan, Ozyurt Birsen, Ozugurlu Fikret, Ozgocmen Salih, Fadillioglu Ersin
Department of Pharmacology, Inonu University Faculty of Medicine, Malatya.
Ann Clin Lab Sci. 2005 Spring;35(2):169-73.
Fish oil contains large amounts of essential omega-3 fatty acids, such as eicosapentaenoic and docosahexaneoic acids, which are building structures of cell membranes. The goal of this study was to elucidate the effects of dietary omega-3 fatty acid supplementation on the oxidant/antioxidant status of erythrocytes in rats. The malondialdehyde (MDA) and nitric oxide (NO) levels and the catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GSH-PX) activities were assayed in erythrocytes of male Wistar albino rats after 30 days of dietary supplementation with fish oil (0.4 g/kg/day). Erythrocyte CAT activity in the treated group was increased in comparison with the control group. Erythrocyte MDA and NO levels were lower in the treated group than the controls. Erythrocyte GSH-Px and SOD activities did not differ significantly in the 2 groups. Negative correlations were found between SOD and CAT activities, and between SOD and GSH-Px activities in the treated group. In conclusion, omega-3 fatty acid supplementation helps to prevent lipid peroxidation and to safeguard erythrocytes from oxidative injury. Dietary supplementation with omega-3 fatty acids might possibly protect tissues from oxygen free radical injury in the various diseases in which the oxidant/antioxidant defense mechanisms are disturbed.
鱼油含有大量必需的ω-3脂肪酸，如二十碳五烯酸和二十二碳六烯酸，它们是细胞膜的组成结构。本研究的目的是阐明膳食补充ω-3脂肪酸对大鼠红细胞氧化/抗氧化状态的影响。在雄性Wistar白化大鼠经30天膳食补充鱼油（0.4 g/kg/天）后，测定其红细胞中的丙二醛（MDA）和一氧化氮（NO）水平以及过氧化氢酶（CAT）、超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-PX）活性。与对照组相比，治疗组的红细胞CAT活性增加。治疗组的红细胞MDA和NO水平低于对照组。两组的红细胞GSH-Px和SOD活性无显著差异。在治疗组中，SOD与CAT活性之间以及SOD与GSH-Px活性之间呈负相关。总之，补充ω-3脂肪酸有助于预防脂质过氧化，并保护红细胞免受氧化损伤。膳食补充ω-3脂肪酸可能有助于在氧化/抗氧化防御机制受到干扰的各种疾病中保护组织免受氧自由基损伤。
