构巢曲霉pH信号转导成分PalC的突变分析支持其与BRO1结构域家族成员存在远缘相似性。
Mutational analysis of the pH signal transduction component PalC of Aspergillus nidulans supports distant similarity to BRO1 domain family members.
作者信息
Tilburn Joan, Sánchez-Ferrero Juan C, Reoyo Elena, Arst Herbert N, Peñalva Miguel A
机构信息
Department of Infectious Diseases, Faculty of Medicine, Imperial College London, United Kingdom.
出版信息
Genetics. 2005 Sep;171(1):393-401. doi: 10.1534/genetics.105.044644. Epub 2005 Jun 8.
Abstract
The alkaline ambient pH signal transduction pathway component PalC has no assigned molecular role. Therefore we attempted a gene-specific mutational analysis and obtained 55 new palC loss-of-function alleles including 24 single residue substitutions. Refined similarity searches reveal conserved PalC regions including one with convincing similarity to the BRO1 domain, denoted PCBROH, where clustering of mutational changes, including PCBROH key residue substitutions, supports its structural and/or functional importance. Since the BRO1 domain occurs in the multivesicular body (MVB) pathway protein Bro1/Vps31 and also the pH signal transduction protein PalA (Rim20), both of which interact with MVB component (ESCRT-III protein) Vps32/Snf7, this might reflect a further link between the pH response and endocytosis.
摘要
碱性环境pH信号转导途径组分PalC的分子作用尚未明确。因此，我们尝试进行基因特异性突变分析，获得了55个新的palC功能缺失等位基因，其中包括24个单残基取代。精细的相似性搜索揭示了保守的PalC区域，其中一个区域与BRO1结构域具有令人信服的相似性，称为PCBROH，包括PCBROH关键残基取代在内的突变变化聚集，支持其结构和/或功能重要性。由于BRO1结构域存在于多囊泡体（MVB）途径蛋白Bro1/Vps31以及pH信号转导蛋白PalA（Rim20）中，这两者都与MVB组分（ESCRT-III蛋白）Vps32/Snf7相互作用，这可能反映了pH反应与内吞作用之间的进一步联系。
相似文献
1
Mutational analysis of the pH signal transduction component PalC of Aspergillus nidulans supports distant similarity to BRO1 domain family members.构巢曲霉pH信号转导成分PalC的突变分析支持其与BRO1结构域家族成员存在远缘相似性。
Genetics. 2005 Sep;171(1):393-401. doi: 10.1534/genetics.105.044644. Epub 2005 Jun 8.
2
PalC, one of two Bro1 domain proteins in the fungal pH signalling pathway, localizes to cortical structures and binds Vps32.PalC是真菌pH信号通路中两种含有Bro1结构域的蛋白质之一，定位于皮质结构并与Vps32结合。
Traffic. 2007 Oct;8(10):1346-64. doi: 10.1111/j.1600-0854.2007.00620.x. Epub 2007 Aug 13.
3
Control of Bro1-domain protein Rim20 localization by external pH, ESCRT machinery, and the Saccharomyces cerevisiae Rim101 pathway.通过外部pH值、内体分选转运复合体（ESCRT）机制和酿酒酵母Rim101途径对Bro1结构域蛋白Rim20定位的调控
Mol Biol Cell. 2006 Mar;17(3):1344-53. doi: 10.1091/mbc.e05-10-0949. Epub 2006 Jan 11.
4
Multivesicular body-ESCRT components function in pH response regulation in Saccharomyces cerevisiae and Candida albicans.多囊泡体-内体分选转运复合体组件在酿酒酵母和白色念珠菌的pH响应调节中发挥作用。
Mol Biol Cell. 2004 Dec;15(12):5528-37. doi: 10.1091/mbc.e04-08-0666. Epub 2004 Sep 15.
5
Ambient pH signal transduction in Aspergillus: completion of gene characterization.曲霉中的环境pH信号转导：基因表征的完成
Mol Microbiol. 1999 Sep;33(5):994-1003. doi: 10.1046/j.1365-2958.1999.01540.x.
6
An ordered pathway for the assembly of fungal ESCRT-containing ambient pH signalling complexes at the plasma membrane.真菌 ESCRT 包含物在质膜处的环境 pH 信号复合物组装的有序途径。
J Cell Sci. 2012 Apr 1;125(Pt 7):1784-95. doi: 10.1242/jcs.098897. Epub 2012 Feb 17.
7
Bro1 is an endosome-associated protein that functions in the MVB pathway in Saccharomyces cerevisiae.Bro1是一种与内体相关的蛋白质，在酿酒酵母的多泡体途径中发挥作用。
J Cell Sci. 2003 May 15;116(Pt 10):1893-903. doi: 10.1242/jcs.00395. Epub 2003 Mar 18.
8
Liaison alcaline: Pals entice non-endosomal ESCRTs to the plasma membrane for pH signaling.碱性衔接蛋白：伴侣蛋白将非内体 ESCRTs 招募到质膜处进行 pH 信号转导。
Curr Opin Microbiol. 2014 Dec;22:49-59. doi: 10.1016/j.mib.2014.09.005.
9
Rescue of Aspergillus nidulans severely debilitating null mutations in ESCRT-0, I, II and III genes by inactivation of a salt-tolerance pathway allows examination of ESCRT gene roles in pH signalling.通过失活耐盐途径来挽救严重削弱 Aspergillus nidulans 的 ESCRT-0、I、II 和 III 基因缺失突变，可研究 ESCRT 基因在 pH 信号中的作用。
J Cell Sci. 2011 Dec 1;124(Pt 23):4064-76. doi: 10.1242/jcs.088344.
10
Analysis of the dual function of the ESCRT-III protein Snf7 in endocytic trafficking and in gene expression.内体分选转运复合体III（ESCRT-III）蛋白Snf7在胞吞运输和基因表达中的双重功能分析。
Biochem J. 2009 Oct 23;424(1):89-97. doi: 10.1042/BJ20090957.
引用本文的文献
1
Mutational analysis of the Aspergillus ambient pH receptor PalH underscores its potential as a target for antifungal compounds.烟曲霉环境pH受体PalH的突变分析突出了其作为抗真菌化合物靶点的潜力。
Mol Microbiol. 2016 Sep;101(6):982-1002. doi: 10.1111/mmi.13438. Epub 2016 Jul 15.
2
ESCRT-III-Associated Protein ALIX Mediates High-Affinity Phosphate Transporter Trafficking to Maintain Phosphate Homeostasis in Arabidopsis.ESCRT-III相关蛋白ALIX介导高亲和力磷酸盐转运体的运输以维持拟南芥中的磷酸盐稳态。
Plant Cell. 2015 Sep;27(9):2560-81. doi: 10.1105/tpc.15.00393. Epub 2015 Sep 4.
3
The vacuolar-sorting protein Snf7 is required for export of virulence determinants in members of the Cryptococcus neoformans complex.液泡分选蛋白Snf7是新型隐球菌复合体成员中致病决定因素输出所必需的。
Sci Rep. 2014 Sep 2;4:6198. doi: 10.1038/srep06198.
4
In Aspergillus nidulans the suppressors suaA and suaC code for release factors eRF1 and eRF3 and suaD codes for a glutamine tRNA.在构巢曲霉中，抑制基因suaA和suaC分别编码释放因子eRF1和eRF3，而suaD编码一种谷氨酰胺tRNA。
G3 (Bethesda). 2014 Apr 11;4(6):1047-57. doi: 10.1534/g3.114.010702.
5
Rescue of Aspergillus nidulans severely debilitating null mutations in ESCRT-0, I, II and III genes by inactivation of a salt-tolerance pathway allows examination of ESCRT gene roles in pH signalling.通过失活耐盐途径来挽救严重削弱 Aspergillus nidulans 的 ESCRT-0、I、II 和 III 基因缺失突变，可研究 ESCRT 基因在 pH 信号中的作用。
J Cell Sci. 2011 Dec 1;124(Pt 23):4064-76. doi: 10.1242/jcs.088344.
6
Calpain chronicle--an enzyme family under multidisciplinary characterization.钙蛋白酶编年史——一个多学科特征的酶家族。
Proc Jpn Acad Ser B Phys Biol Sci. 2011;87(6):287-327. doi: 10.2183/pjab.87.287.
7
Self-protection against gliotoxin--a component of the gliotoxin biosynthetic cluster, GliT, completely protects Aspergillus fumigatus against exogenous gliotoxin.自身抵御曲霉菌毒素——曲霉菌毒素生物合成簇的一个组成部分GliT，能完全保护烟曲霉抵御外源曲霉菌毒素。
PLoS Pathog. 2010 Jun 10;6(6):e1000952. doi: 10.1371/journal.ppat.1000952.
8
Characterization of Aspergillus nidulans DidB Did2, a non-essential component of the multivesicular body pathway.鉴定构巢曲霉 DidB（Did2），多泡体途径的非必需组分。
Fungal Genet Biol. 2010 Jul;47(7):636-46. doi: 10.1016/j.fgb.2010.03.010. Epub 2010 Apr 1.
9
Mutational analysis of Candida albicans SNF7 reveals genetically separable Rim101 and ESCRT functions and demonstrates divergence in bro1-domain protein interactions.对白色念珠菌 SNF7 的突变分析揭示了遗传上可分离的 Rim101 和 ESCRT 功能，并证明了 BRO1 结构域蛋白相互作用的差异。
Genetics. 2010 Mar;184(3):673-94. doi: 10.1534/genetics.109.112029. Epub 2009 Dec 21.
10
Systemic analysis of the response of Aspergillus niger to ambient pH.黑曲霉对环境pH响应的系统分析
Genome Biol. 2009;10(5):R47. doi: 10.1186/gb-2009-10-5-r47. Epub 2009 May 1.
本文引用的文献
1
The Aspergillus pH-responsive transcription factor PacC regulates virulence.烟曲霉pH响应转录因子PacC调节毒力。
Mol Microbiol. 2005 Feb;55(4):1072-84. doi: 10.1111/j.1365-2958.2004.04472.x.
2
Snf7p, a component of the ESCRT-III protein complex, is an upstream member of the RIM101 pathway in Candida albicans.Snf7p是内体分选转运复合体Ⅲ（ESCRT-III）蛋白复合物的一个组成部分，是白色念珠菌中RIM101信号通路的上游成员。
Eukaryot Cell. 2004 Dec;3(6):1609-18. doi: 10.1128/EC.3.6.1609-1618.2004.
3
Protein homology detection by HMM-HMM comparison.通过隐马尔可夫模型（HMM）比较进行蛋白质同源性检测。
Bioinformatics. 2005 Apr 1;21(7):951-60. doi: 10.1093/bioinformatics/bti125. Epub 2004 Nov 5.
4
Recent advances in the characterization of ambient pH regulation of gene expression in filamentous fungi and yeasts.丝状真菌和酵母中基因表达的环境pH调节特性研究的最新进展。
Annu Rev Microbiol. 2004;58:425-51. doi: 10.1146/annurev.micro.58.030603.123715.
5
Multivesicular body-ESCRT components function in pH response regulation in Saccharomyces cerevisiae and Candida albicans.多囊泡体-内体分选转运复合体组件在酿酒酵母和白色念珠菌的pH响应调节中发挥作用。
Mol Biol Cell. 2004 Dec;15(12):5528-37. doi: 10.1091/mbc.e04-08-0666. Epub 2004 Sep 15.
6
Bro1 coordinates deubiquitination in the multivesicular body pathway by recruiting Doa4 to endosomes.Bro1通过将Doa4招募至内体来协调多泡体途径中的去泛素化作用。
J Cell Biol. 2004 Aug 30;166(5):717-29. doi: 10.1083/jcb.200403139. Epub 2004 Aug 23.
7
MEGA3: Integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment.MEGA3：用于分子进化遗传学分析和序列比对的集成软件。
Brief Bioinform. 2004 Jun;5(2):150-63. doi: 10.1093/bib/5.2.150.
8
Genome evolution in yeasts.酵母中的基因组进化
Nature. 2004 Jul 1;430(6995):35-44. doi: 10.1038/nature02579.
9
CHMP4b is a major binding partner of the ALG-2-interacting protein Alix among the three CHMP4 isoforms.在三种CHMP4亚型中，CHMP4b是与ALG-2相互作用蛋白Alix的主要结合伴侣。
Arch Biochem Biophys. 2004 Jan 1;421(1):159-65. doi: 10.1016/j.abb.2003.09.038.
10
Overlap of nuclear localisation signal and specific DNA-binding residues within the zinc finger domain of PacC.PacC锌指结构域内核定位信号与特定DNA结合残基的重叠
J Mol Biol. 2003 Dec 5;334(4):667-84. doi: 10.1016/j.jmb.2003.09.072.
Zotero 插件